560 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



0.3% glucose and 1.0 cc. dye per 100.0 cc. 

 agar. 

 Variants : 



(a) Makgill added 1.0% of a saturated 

 solution of neutral red to glucose 

 agar. 



(b) Heinemann added sufficient 0.5% 

 neutral red to give a red color to 1.0% 

 glucose agar. 



(c) Bezangon prepared a similar medium 

 as follows: 



(1) Dissolve 5.0 g. of agar in 1000.0 cc. 

 of bouillon. 



(2) To melted (1) nearly solid, add 2.0 

 g. glucose and 0.7 cc. of a aqueous 

 saturated solution of neutral red. 



(3) Tube. 



(4) Sterilization not specified. 



(d) Rothberg and Scheffler (Klimmer) 

 prepared a medium as follows: 



(1) Add 0.3% glucose to melted sterile 

 nutrient agar. 



(2) Add 1.0 cc. of a saturated (cold) 

 solution of neutral red sterilized in 

 the steamer to each 100.0 cc. of (1). 



(e) Cunningham prepared a medium as 

 follows: 



(1) Add 1.5% agar to bouillon. 



(2) Steam for 30 minutes to dissolve 

 the agar. 



(3) Boil over an open flame for 15 

 minutes, stirring constantly. 



(4) Adjust the reaction to a slight 

 alkalinity using turmeric paper as 

 an indicator (distinctly brown). 



(5) Filter while hot thru a plug of clean 

 cotton-wool in the bottom of an 

 enamelled funnel. 



(6) Add 0.5% glucose dissolved in a 

 few cc. of water. 



(7) Stir to thoroly mi.x. 



(8) Tube. 



(9) Sterilize intermittently in steam. 

 References: SchefHer (1900 p. 202), Magkill 



(1901 p. 431), Heinemann (1905 p. 127), 

 Tanner (1919 p. 50), Bezangon (1920 p. 

 114), Klimmer (1923 p. 211), Cunningham 

 (1924 p. 16), Savage (1901 p. 437), Irons 

 (1902 p. 315). 



1791. Martin and Loiseau's Glucose Litmus 



Agar 

 Constituents : 



1. Distilled water 200.0 cc. 



2. Glucose 3.0 g. 



3. Litmus (soln.) 30.0 cc. 



4. Nutrient agar (3.0%) 

 Preparation : 



(1) Dissolve 3.0 g. of glucose and 30.0 

 cc. of litmus in 200.0 cc. distilled 

 water. 



(2) Add five parts sterile (1) to six parts 

 of sterile nutrient 3.0% agar in tubes. 

 The mixture should be a violet tint. 



Sterilization: Sterilize (1) by filtering. 

 Method of sterilization of 3.0% agar not 

 given. 



Use: Differentiation of diphtheria and 

 pseudo diphtheria. True diphtheria 

 bacilli changed the color of the medium 

 to red and grew only anaerobically. 

 Pseudodiphtheria strains did not change 

 the color and grew only on the surface. 



Reference: Martin and Loiseau (1919 p. 

 73). Taken from (1919 p. 185). 



1792, Frost's Lactose Agar 



Constituents : 



1. Nutrient agar 1000.0 cc. 



2. Lactose (1.0%) 10.0 g. 



Preparation : 



(1) Add 1.0% lactose to nutrient agar. 



(2) Tube. 



Sterilization: Sterilize in the steamer. 

 Use: General culture medium. 

 Reference: Frost (1903 p. 64). 



1793. Wurtz's Litmus Lactose Agar 



Constituents : 



1. Infusion agar 1000.0 cc. 



2. Lactose (2.0%) 20.0 g. 



3. Litmus 

 Preparation : 



(1) Add 2.0% lactose to sterile infusion 

 agar. 



(2) Tube. 



(3) Melt sterile (2) and add suflicient 

 tincture of litmus to give a violet 

 color. 



(4) Pour sterile (3) into plates. 

 Sterilization: Method of sterilization of 



(1) or (2) not given. Sterilize (3) at 

 100 °C. 

 Use: Differentiation of Bad. coli and 

 Eberth's bacillus. Author reported that 

 Bad. coli fermented lactose with the 

 production of acid. Typhoid bacillus 

 colonies were colorless, colon colonies 

 were red. 



