562 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



(7) Add 2.0% lactose and 2.0% 

 peptone to hot (6) and mix 

 until solution is complete. 



(8) Add (2) t6 (7). 



(9) Adjust the reaction to 0. 



(10) Add 2.0% azolitmin solution. 



(11) Boil over a free flame. 



(12) Distribute in 100.00 cc. lots in 

 250.0 cc. Florence flasks. 



(13) Sterilization not specified. 

 References: Wurtz (1897 p. 43), Smith 



(1905 p. 94), Heinemann (1905 p. 127), 

 Committee American Public Health (1913 

 p. 129), Ball (1919 p. 78), Abbott (1921 p. 

 142), Giltner (1921 pp. 379, 380). 



1794. Gassner's Metachrome Yellow Water 

 Blue Lactose Agar (Klimmer) 



Constituents : 



1. Nutrient agar 2000.0 cc. 



2. Metachrome yellow (2.0% 



soln.) 125.0 cc. 



3. Water blue (6B extra 



"Afga" 1.0% soln.) 175.0 cc. 



4. Lactose 100.0 g. 



Preparation : 



(1) Make two liters of agar prepared from 

 yeast or meat and peptone, slightly 

 alkaline. 



(2) Add 125.0 cc. of a 2.0% solution of 

 metachrome yellow (boiled two 

 minutes). 



(3) Dissolve 100.0 g. lactose in 175.0 cc. 

 of a 1.0% solution of water blue (6B 

 extra "Afga") and boil 10 minutes. 



(4) Add (3) to (2). 

 Sterilization: Not specified. 



Use: Detection of typhoid bacilli. Author 

 reported that B. coli colonies were deep 

 blue. Typhoid and dysentery colonies 

 lightened up the medium, coloring it a 

 greyish yellow. 



Reference: Klimmer (1923 p. 216). 



1795. Hirschbruch and Schwer's Azolitmin 

 Crystal Violet Lactose Agar 



Constituents: 



1. Nutrient agar (1.0 to 1.5%). 1000.0 cc. 



2. Lactose 15.0 g. 



3. Crystal violet (0.1%, soln.) . 10.0 cc. 



4. Azolitmin 0.4 g. 



Preparation : 



(1) Prepare nutrient agar containing 1.0 

 to 1.5% agar. 



(2) Place 1.5 g. of lactose into a sterile 

 flask. 



(3) Pour approximately 100.0 cc. of the 

 liquid agar over the lactose and mix 

 well. 



(4) Boil 100.0 g. of distilled water 15 

 minutes. 



(5) Dissolve 0.1 g. crystal violet in (4). 



(6) Add a weak soda solution that has 

 been boiled several times to (3) until 

 the desired degree of alkalinity is 

 obtained. 



(7) Dissolve 0.04 g. azolitmin in a little 

 water and boil. 



(8) Add 1.0 cc. of (5) and the azolitmin 

 solution to (6) (the melted lactose 

 agar) that has been cooled to 45°C. 



(9) Mix well and after about ten minutes, 

 pour into sterile Petri dishes. Allow 

 the agar to solidify with the covers 

 removed. 



Sterilization: Method not given. 



Use: Diagnosis of cholera. Authors re- 

 ported that cholera colonies were deep 

 blue. 



Reference: Hirschbruch and Schwer 

 (1904 p. 150). 



1796. Ramond's Rubine Acid Lactose Agar 



Constituents : 



1. Nutrient agar 1000.0 cc. 



2. Lactose 40.0 g. 



3. Eubine acid 

 Preparation : 



(1) Add 4 parts per 100 of lactose to 

 nutrient agar. 



(2) Add several grains of rubine acid 

 until the medium is colored red. 



(3) Heat to 70 or 80°C. and add a satu- 

 rated solution of Na2C03 until the 

 color disappears. 



(4) Filter thru paper. 



(5) Distribute in tubes. 

 Sterilization: Sterilize at 115°C. for five 



minutes. 

 Use : Detection of typhoid bacilli. Author 



reported that Bad. coli colonies colored 



the medium red. Typhoid colonies did 



not change the color. 

 References: Ramond (1896 p. 884), Wurtz 



(1897 p. 43). 



1797. Delta's Fuchsin Lactose Agar 



Constituents : 



1. Distilled water. 



.0 cc. 



