566 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



favored the adherence of the agar to 

 the walls of the containing vessels. 

 Reference: Wurtz (1897, p. 46), Glucks- 

 mann (1897 p. 436), Thoinot and Masselin 

 (1902 p. 35), Smith (1902 p. 92), Dalton 

 and Eyre (1904 p. 159), Heinemann (1905 

 p. 128), Smith (1905 p. 196), Roux and 

 Rochaix (1911 p. 117), Roddy (1917 p. 43), 

 Ball (1919 p. 74), Besson (1920 p. 43), 

 Dopter and Sacquepee (1921 p. 128), 

 Bitfield (1922 p. 117), Stitt (1923 p. 38), 

 Cunningham (1924 p. 165) . 



1809 



. Scheffler's Indicator Glycerol Agar 



Constituents : 



1. Glycerol agar 1000.0 cc. 



2. Neutral red (Concentrated 

 aqueous soln.) 15.0 cc. 



Preparation : 



(1) To 100.0 cc. of glycerol agar add 1.5 

 cc. of a concentrated watery solution 

 of neutral red. 



Sterilization: Not specified. 



Use: Detection of iBac^m'MW coli. Author 

 reported that after 24 hours in^ neutral 

 red, a slight greenish coloration was 

 formed, in 48 hours a distinct green 

 fluorescence. At the end of 24 hours with 

 saffarin, the under surface was lighter 

 and after 48 hours decolorization occurred. 



Variants: The author substituted a con- 

 centrated watery solution of saffarin for 

 neutral red. 



Reference: Scheffler (1900 p. 201). 



1810. Mandelbaum's Rosolic Acid Glycerol 

 Agar 



Constituents : 



1. Glycerol agar 6.0%. 



2. Rosolic acid 1.0% alcoholic soln. 

 Preparation : 



(1) Brepare a 6.0% glycerol agar. 



(2) Brepare a 1.0% alcoholic solution of 

 rosolic acid. 



(3) Melt sterile (1), cool to 50°C. and to 

 each tube add 0.3 cc. of (2). 



Sterilization: Not specified. 



Use: Differentiation of typhoid and meta- 

 typhoid bacilli. Author reported that 

 metatyphoid bacilli gave red colonies. 

 The typhoid colonies were yellow. 



Reference: Mandelbaum (1912 p. 48). 



1811. Heinemann's Litmus Mannitol Agar 



Constituents : 



1. Sugar free agar 1000.0 cc. 



2. Mannitol (1.0%) 10.0 g. 



3. Litmus 

 Preparation : 



(1) Add 1.0% mannite to sugar-free agar. 



(2) Tube in 8.0 cc. quantities. 



(3) Add 1.0 cc. of 1.0% sterile litmus 

 solution to each tube before use. 



Sterilization: Not specified. 

 Use: General culture medium. 

 Reference: Heinemann (1905 p. 127). 



1812. Penfold's Phenylacetate Agar 

 Constituents : 



1. Nutrient agar 150.0 cc. 



2. Bhenylacetate (10.0% soln.) 

 Preparation : 



(1) Dissolve phenylacetate in saturated 

 Na2C03 solution until the solution is 

 just faintly alkaline to neutral litmus 

 paper. 



(2) Add distilled water to render the 

 solution of 10.0%, strength. 



(3) Filter thru a Doulton filter. 



(4) Pour ascending quantities of the 

 solution into Petri dishes and 15.0 cc. 

 of melted agar added to each plate. 



Sterilization: Not given. 



Use : To show inhibition of bacterial growth 

 using members of the colon-aerogenes 

 group. Author reported that B. coli 

 produced papillated colonies and showed 

 marked variability in the size of its 

 colonies. 



Reference: Benfold (1913-14 p. 38). 



1813. Wurtz's Phenol Agar (Copeland) 

 Constituents : 



1. Wurtz agar. 



2. Phenol 2.0% solution. 

 Preparation: 



(1) Method of preparation or composition 

 of Wurtz agar not given. 



(2) Add 0.2 cc. of a 2.0% solution of car- 

 bolic acid to the agar. Amount of 

 agar not specified. 



Sterilization: Not specified. 



Use: Isolation of Bacillus coli communis 



from water. 

 Variants: Behmer's (Klimmer) added 2.0 



cc. of a 5.0% phenol solution to each 



100.0 cc. agar. 



