CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 567 



Reference: Copeland (1901 p. 493), Klim- Use: CultivatioQ of typhoid and inter- 



mer (1923 p. 227). mediate group. 



Reference: Hurler (1912 p. 357). 

 1814. Penfold's Monochlorhydrin Agar 



p , , 1817. Finger, Ghon and Schlagenhaufer's 



, , " Urea Agar 



1. Agar. ^ 



2. Monochlorhydrin 20.0% solution. Constituents: 



Preparation: 1. Nutrient agar (2.0% agar, 



(1) Prepare a 20.0% solution of mono- 1.0% peptone) 1000.0 cc. 



chlorhydrin. 2. Urea (2.0%) 20.0 g. 



(2) Place ascending quantities of sterile Preparation: 



(1) in Petri dishes. (1) Prepare nutrient agar containing 



(3) Add 15.0 cc. of sterile agar to each 2.0% agar with 1.0% peptone, 

 plate. Mi.xwell. (2) Add 2.0% urea to (1). 



Sterilization: Filter (1) to sterilize. Sterilization: Not specified. 



Method of sterilization of agar not given. Use: Cultivation of gonococci. Author 



Use : To show inhibition of bacterial growth reported that growth was not as luxuriant 



using members of colon aerogenes group. as in serum agar. Also used by other in- 



Author reported that B. coli produced vestigators to study urea decomposition. 



various sized colonies and papillae may Variants: 



be formed. (a) The author used 3.0 or 5.0% urea 



Reference: Penfold (1913-14 p. 39). instead of 2.0%. 



(b) Lohnis studied urea decomposition 



1815. Jacobson's Ethylcinnamic Ether Agar ^^ ^^^.^^ ^ ^^ ^^ ^ J5 0^^ ^q^^^^^ 



Constituents: solution of urea to each tube of 



1. Agar. nutrient agar. The medium was then 



2. Ethylcinnamic ether. heated in the steam sterilizer. 

 Preparation: (c) Cunningham added 2.0, 5.0 or 10.0% 



(1) Liquify 10.0 cc. tubes of nutrient agar urea to nutrient agar, and sterilized 

 by heating on the salt water bath. by heating intermittently in the 



(2) Add one small drop of ethyl cinnamic steamer. The medium was used to 

 ether to each tube by means of a fine study urea decomposition, 

 pipette. This is equivalent to 0.025 g. Reference: Finger, Ghon and Schlagen- 



Sterilization: Not specified. haufer (1894 p. 14), Lohnis (1913 p. 95), 



Use: Differentiation of dysentery bacilli. Cunningham (1924 p. 143). 



Author reported that the Hiss type of -^^ ,,. -^ ■, , r^ 



, . r -1 J 4. J 1 ■ 4.U 1818. Russell's Double Sugar Agar 



dysentery failed to develop in the pres- ^ ^ 



ence of ethyl cinnamic ether while the Constituents: 



Flexner showed good development. 1. Nutrient agar (2.0 or 



Reference : Jacobson (1919 p. 726). 3.0%) 1000.0 cc. 



2. Litmus solution 



1816. Hurler's Caffeine Agar ^^ 0^^ ^q^^^^^ ^^1^. 



Constituents: tion) 30.0 to 50.0 cc. 



1. Nutrient agar 1000.0 cc. 3. Lactose 10.0 g. 



2. Caffeine (0.3%) 3.0 g. 4. Glucose 1.0 g. 



Preparation : Preparation : 



(1) Prepare nutrient agar. (1) Prepare2.0or 3.0% nutrient agar with 



(2) Adjust the reaction of (1) to slightly a reaction of about 0.8% acid to 

 alkaline. phenolphthalein. 



(3) Add 0.3% caffeine to (2). The (2) Add enough of a 5.0% aqueous solu- 

 caffeine is dissolved in 5.0 cc. of tion of litmus to (1) to give a distinct 

 distilled water by heating over the purple violet, the amount required 

 water bath before adding to the agar. depending on the original color of the 



Sterilization: Method not given. agar. 



