CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



569 



1821. Kligler and Defandorfer's Double 



Sugar Agar 



Prepared in the same manner as Russell's 

 Double Sugar Medium (see 1818) using the 

 same constituents but substituting 0.5% 

 mannitol for 1.0% lactose. The authors 

 reported they used the medium for the 

 differentiation of B. typhosus from dys- 

 entery bacilli. 

 Reference: Kligler and Defandorfer (1918 



p. 439). 



1822. Kendall and Ryan's Sucrose Mannitol 



Agar 



Constituents : 



1. Nutrient agar 2.5% 1000.0 cc. 



2. Mannitol 1.0 g. 



3. Sucrose, 10.0 g. 



4. Andrade indicator 10.0 cc. 



Preparation : 



(1) Prepare nutrient 2.5% agar. 



(2) Adjust to a reaction so that when 

 Andrade indicator is added that the 

 medium be a faint pink while hot. 



(3) Add 2 and 3 to (2). 



(4) Tube. 



(5) Slant so that slanted surface begins 

 1.0 cm. from the bottom. Medium is 

 colorless when cold. 



Sterilization: Not specified. 



Use: Detection of intestinal and other 



bacteria. 

 Reference : Kendall and Ryan (1919 p. 403). 



1823. Hulton-Frankel and MacDonald's 

 Inositol-Dextrin Agar 



Constituents : 



1. Nutrient agar (3.0%) 1000.0 cc. 



2. Inositol (1.0%) 10.0 g. 



3. Dextrin (1.0%) 10.0 g. 



4. Litmus 

 Preparation : 



(1) Add 1.0% inositol and 1.0% dextrin 

 to 3.0% nutrient agar. 



(2) Add litmus as an indicator. 

 Sterilization: Not specified. 



Use: Differentiation of typhoid group. 

 The authors reported that typhoid 

 fermented dextrin with acid formation in 

 the butt of the tube, decolorizing entirely 

 in 24 hours with a violet slant. Para- 

 typhoid A did not ferment either dextrin 



or inositol. Paratyphoid B fermented 

 inositol with the formation of gas. 

 Reference: Hulton-Frankel and Mac- 

 Donald (1917 p. 31). 



1824. Kitasato's Glucose Formate Agar 

 (Tanner) 



Constituents : 



1. Nutrient agar 1000.0 cc. 



2. Glucose 20.0 g. 



3. Sodium formate 4.0 g. 



Preparation : 



(1) Prepare nutrient agar. 



(2) Dissolve 2 and 3 in 1. 



(3) Adjustment of reaction not given. 



(4) Tube. 



Sterilization: Method not given. 

 Use: General culture medium. 

 Reference: Tanner (1919 p. 49). 



1825. Sohngen and Fol's Glucose Butyrate 

 Agar 



Constituents : 



1. Water agar 50.0 cc. 



2. Nutrient agar 50.0 cc. 



3. Calcium butyrate 1.0 g. 



4. Glucose 0.25 g. 



Preparation : 



(1) Prepare tap water agar and nutrient 

 agar. 



(2) Mix 50.0 cc. of tap water and 50.0 cc. 

 of nutrient agar. 



(3) Dissolve 3 and 4 in (2). 

 Sterilization: Not specified. 



Use: Cultivation of actinomyces, Acti- 

 nomyces elastica and Actinomyces fuscus 

 Reference: Sohngen and Fol (1914 p. 95). 



1826. MacDonald's Glucose Lactic Acid 

 Agar 



Constituents : 



1. Nutrient agar 1000.0 cc. 



2. Glucose 10.0 g. 



3. Lactic acid 1.0 to 10.0 g. 



Preparation : 



(1) Prepare nutrient agar using Armour's 

 peptone. 



(2) Titrate carefully so that after one 

 hour sterilization in the autoclave at 

 15 pounds pressure, the reaction is 

 neutral to phenolphthalein. 



(3) Tube in 10.0 cc. lots. 



(4) Cool sterile (3) to 50°C. and add 

 lactic acid in varying amounts to 



