570 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



each tube undiluted so that 0.1 cc. 

 equals 1.0% of acid. 

 (5) Add glucose. 



Sterilization: Sterilize in autoclave at 15 

 pounds pressure one hour. 



Use : To show effect of lactic acid on growth 

 of organisms. Author reported that lactic 

 acid had a varying degree of inhibitory 

 action on bacteria and fungi. Generally 

 1.0% lactic acid inhibited growth. 



Reference: MacDonald (1917 p. 322). 



1827. Miiller's Lactose Tartrate Agar 



Constituents : 



1. Bouillon 1000.0 cc. 



2. Lactose 25.0 g. 



3. Agar 30.0 g. 



4. Potassium ferric tartrate 



5. Potassium ferrocyanide 



6. Fuchsin 

 Preparation : 



(1) Dissolve 30.0 g. of agar and 25.0 g. 

 lactose in 1 liter of bouillon. 



(2) Neutralize and then add 10.0 to 12.0 

 cc. of normal NaOH to (1). 



(3) Prepare a 10.0% solution of potassium 

 ferrocyanide. 



(4) Prepare a 10.0% solution of potassium 

 ferric tartrate. 



(5) Add 50.0 cc. of sterile (4) to 1000.0 cc. 

 of sterile (2). Mix well. 



(6) Add 50.0 cc. of sterile (3) to (5). Mix 

 well. 



(7) Color the agar by the addition of 

 10.0 cc. fuchsin solution. 



(8) Distribute as desired in sterile con- 

 tainers. 



Sterilization: Sterilize (2) in the usual 

 manner (method not given) . The lactose 

 may be sterilized at 100°C. in water and 

 then added to the sterile agar if desired. 

 Sterilize (3) by heating at 100° for 30 

 minutes. Sterilize (4) by heating at 

 100 °C. for 30 minutes. 

 Use: Differentiation of colon typhoid 

 bacilli. The author gave the following 

 reactions: 



With fuchsin, typhoid blue; coli red. 

 With safranin, typhoid blue; coli orange. 

 With Bismark brown, typhoid brown, 



coli bright green. 

 With G. orange, typhoid brown, coli 



bright green. 



Variants: The author used one of the 

 following instead of 10.0 cc. of fuchsin: 



Safranin 10.0 cc. 



Bismark brown 50.0 cc. 



G. orange 60.0 cc. 



Neutral red 60.0 cc. 



Reference: Miiller (1922 p. 1251). 



1828. Kligler's Lead Acetate Glucose Agar 



Constituents : 



1. Nutrient agar (0.5%). 1000.0 cc. 



2. Glucose (0.2%) 2.0 g. 



3. Lead acetate (0.05 to 



0.1%) 0.5 to 1.0 g. 



Preparation : 



(1) Add 0.05% to 0.1% lead acetate and 

 0.2% glucose to 0.5% nutrient agar. 



Sterilization: Method not given. 



Use: Differentiation of typhoid and para- 

 typhoid bacilli. The author reported 

 that B. typhi produced browning without 

 gas. B. paratyphi B. produced browning 

 with gas, B. paratyphi A. produced no 

 browning and gas and B. dysenteriae 

 produced no browning and no gas. 



Reference: Kligler (1917 p. 805). 



1829. MacConkey's Lactose Bile Salt Agar 

 (Heinemann) 



Constituents : 



1. Nutrient agar 100.0 cc. 



2. Sodium taurocholate (0.5%). 0.5 g. 



3. Peptone (2.0%) 2.0 g. 



4. Lactose (2.0%) 2.0 g. 



Preparation : 



(1) Prepare nutrient agar. 



(2) Add 2 and 3 to (1). 



(3) Boil. 



(4) Clarify. 



(5) Filter. 



(6) Add 2.0% lactose. 



(7) Tube. 



Sterilization: Method not given. 

 Use: General culture medium. 

 Reference: Heinemann (1905 p. 128). 



1830. Fleming's Oleic Acid Glycerol Agar 



Constituents : 



1. Nutrient agar 100.0 cc. 



2. Glycerol (2.0%) 2.0 g. 



3. Oleic acid (0.1%) 0.1 g. 



Preparation : 



(1) Neutralize nutrient agar with HCl. 



