CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



571 



(2) Add 2.0% glycerol and 0.1% oleic 

 acid to (1). 



(3) Tube. 



Sterilization: Sterilize in the autoclave in 

 the usual way. (Details not given.) 



Use: Cultivation of the bacillus of acne 

 vulgaris (Bacillus acne). 



Reference: Fleming (1909 p. 1036). 



1831. Rosenow and Towne's Ascitic Fluid 

 Kidney Agar 



Constituents : 



1. Water 1000.0 cc. 



2. Peptone (Witte's) 



3. Meat extract (Liebig's) 



4. Glucose 10.0 g. 



5. Ascitic fluid (10.0%) 100.0 cc. 



6. Rabbit kidney, sterile 



7. Agar 25.0 g. 



Preparation : 



(1) Prepare an extract broth from water, 

 Liebig's Beef Extract and Witte's 

 peptone (amounts not given). 



(2) Titrate and adjust the reaction of 

 (1) to 0.6-0.8 acid to phenolphthalein. 



(3) Add and dissolve 1.0% glucose and 

 2.5% agar in (2). 



(4) Add 10.0% sterile ascitic fluid to 

 sterile (3). 



(5) Distribute in tall test tubes (column 

 of medium measuring 0.8 cc. in 

 diameter and 13.0 cc. in height. 



(6) Add a piece of fresh sterile rabbit 

 kidney. 



(7) Add a layer of sterile mineral oil. 

 Sterilization: Method of sterilization of 



(3) not given. 



Use: Cultivation of pleomorphic strepto- 

 cocci causing poliomyelitis. The author 

 reported that initial growth could best 

 be obtained without the addition of the 

 kidney. 



Variants: The authors prepared the 

 medium as indicated in step (1) thru (4), 

 omitting the agar. One part of this 

 ascitic fluid broth was mixed with 1.5% 

 agar, tubed and a piece of kidney added 

 to each tube. A layer of sterile mineral 

 oil was added to each tube. 



Reference: Rosenow and Towne (1917 p. 

 177). 



1832. Rosenow's Glucose Brain Agar 

 (Haden) 



Constituents : 



1. Water 1000.0 cc. 



2. Bacto nutrient broth 8.0 g. 



3. NaCl 8.0 g. 



4. Glucose (c.p.) 2.0 g. 



5. Andrade indicator 10.0 cc. 



6. Brain, calf 



7. Marble 



8. Agar 7.0 g. 



Preparation : 



(1) Dissolve 2, 3 and 8 in 1. 



(2) Cool and add 4 and 5. 



(3) Tube in 6 by 0.75 inch test tubes so 

 that the depth is at least 3.4 to 4.0 

 inch. 



(4) Add 3 pieces of crushed calf brain 

 about 1 cmm. square and two or three 

 pieces of crushed marble to each tube. 



Sterilization: Autoclave for 20 minutes at 



15 pounds pressure. 

 Use: Isolation of bacteria from infected 



teeth. 

 Reference: Haden (1923 p. 831). 



1833. Goldberger's Glucose Alkaline Egg 

 Agar (Abbott) 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Egg 1.0 



3. Meat extract (Liebig's) 3.0 g. 



4. Peptone (Witte's) 10.0 g. 



5. NaCl (c.p.) 5.0 g. 



6. Glucose 1.0 g. 



7. Agar 30.0 g. 



Preparation : 



(1) Mix a whole egg with an equal 

 volume of distilled water. 



(2) Mix one volume of (1) with an 

 equal volume of 6.5% solution of 

 anhydrous NajCOa. 



(3) Steam for 30 to 60 minutes. 



(4) Mix 3, 4, 5, 6 and 7 in 1000.0 cc. of 

 water. 



(5) Steam for 3 hours to insure complete 

 solution. 



(6) Decant or filter thru cotton. 



(7) Distribute in 100.0 cc. quantities in 

 flasks. 



(8) Mix one volume of (3) with five 

 volumes of sterile (7). The agar 



