572 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



should be completely liquified by 

 steam. 

 (9) When thoroly mixed, pour into 



Petri dishes. 

 (10) Place in the incubator with covers 

 partially removed. 

 Sterilization: Sterilize (7) by steaming for 



90 minutes. 

 Use: Diagnosis of cholera. Abbott re- 

 ported that cholera vibrio and cholera- 

 like organisms grew luxuriantly while 

 other organisms were restrained. 

 Variants: Krumwiede (Park, Williams and 

 Krumwiede) prepared a similar medium 

 as follows: 



(1) Mix equal parts egg and water. 



(2) Mix equal parts (1) and a 12.0% 

 Na2C03 (crystalline) solution. 



(3) Steam in the Arnold sterilizer for 20 

 minutes. 



(4) Prepare a 3.0% beef extract peptone 

 (Fairchild's) agar. Do not adjust 

 the reaction. The size of the colonies 

 is increased if 0.2% glucose be added. 



(5) Mix 20 parts (3) to 80 parts (4). 

 Sterilization not specified. 



(6) Pour in plates. 



(7) Dry for 20 minutes. 



Reference: Abbott (1921 p. 571), Park, 

 Williams and Krumwiede (1924 p. 130). 



1834. Goldberg's Meat Infusion Extract 

 Agar (Stitt) 



Constituents : 



1. Extract agar (3.0%) 1500.0 cc. 



2. Water 500.0 cc. 



3. Beef 500.0 g. 



Preparation : 



(1) Treat 500.0 g. lean beef with 500.0 g. 

 water. Temperature not specified. 



(2) After 3 hours strain. 



(3) Adjust the reaction to neutral by the 

 addition of 5.3% anhydrous Na2C03 

 solution. 



(4) Add 2.5 cc. of 5.3% anhydrous NaoCOa 

 solution for each 100.0 cc. of infusion. 



(5) Filter sterile (4). 



(6) Prepare a 3.0% meat extract agar. 



(7) Mix one volume of (5) with 3 volumes 

 of hot melted sterile (6). 



(8) Pour plates. 



(9) Cover the plates with a piece of filter 

 paper and place in the incubator for 

 30 minutes until they are dry. 



Sterilization: Sterilize (4) in the Arnold for 

 30 minutes. Sterilization of extract agar 

 not specified. 



Use: Cultivation of cholera organisms. 

 Stitt reported that cholera colonies 

 were clear, round and showed a brownish 

 center. They did not show the striking 

 bluish opalescence as on ordinary plates. 



Reference: Stitt (1923 p. 50). 



1835. Dimitroff's Egg Agar 



Constituents : 



1. Extract agar 750.0 cc. 



2. Extract broth 250.0 cc. 



3. Egg white 

 Preparation : 



(1) Place a small cube of hard boiled egg 

 white in 10.0 cc. of beef extract broth. 



(2) Following sterilization of (1), mix 

 25.0 cc. of (1) in 75.0 cc. of beef extract 

 agar. 



Sterilization: Method of sterilization not 

 given. 



Use: Cultivation of Spirillum virginianum, 

 The author reported that the spirilla 

 produced small dew-drop, convex, glisten- 

 ing, slightly opaque colonies. 



Reference: Dimitroff (1926 p. 22). 



1836. Dunschmann's Bile Salt Gelatin Agar 



Constituents : 



1. Meat extract 1000.0 cc. 



2. Agar (3.0 to 4.0%) . . . 30.0 to 40.0 g. 



3. Gelatin (0.5%) 5.0 g. 



4. Sodium taurocholate 



(1.5 to 2.5%) 15.0 to 20.0 g. 



5. Lactose (4.0%) 40.0 g. 



6. Peptone (5.0%) 50.0 g. 



7. Litmus 

 Preparation : 



(1) Prepare a meat extract with Liebig's 

 beef extract (or by extracting 500.0 g. 

 of veal with 1 liter of water). 



(2) Dissolve 2, 3, 4, 6 and 6 in (1). 



(3) Distribute in flasks or Petri dishes 

 and add 10.0% sensitive litmus 

 (strength of solution not specified). 



Sterilization: Not specified. 



Use: Enrichment medium for typhoid 

 bacilli. Author reported that mineral 

 salts may be used as a basis for this 

 medium instead of extract or infusion 

 broth. 



Reference: Dunschmann (1909 p. 64). 



