586 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



form and preserve in the ice chest 

 for four weeks or sterilize in flowing 

 steam at 100°C. for 30 minutes. 

 Reference: Meyer (1925 p. 47). 



1866. Farcy and Chavaillon's Egg Albumin 

 Serum Agar 



Constituents : 



1. Infusion agar 



2. Serum 100.0 cc. 



3. Egg white 20.0 cc. 



Preparation: 



(1) Add 100.0 cc. of horse serum obtained 

 under aseptic conditions to a medium 

 sized sterile flask containing glass 

 beads. 



(2) Add 20.0 cc. of egg white to (1). 

 Remove the egg white by means of a 

 sterile pipette, and add to (1) under 

 aseptic conditions. 



(3) Shake the flask vigorously for 5 to 10 

 minutes. 



(4) Prepare a 2.5% infusion agar. 



(5) Exactly neutralize (4) and add 0.2 

 cc. of a 10.0% soda solution per liter 

 of agar. 



(6) Mix one part (3) with 3 parts (5), 

 melted and cooled to 50°C. 



(7) Distribute as desired. 

 Sterilization: Not specified. 

 Use: Cultivation of meningococci. 

 Variants: Authors suggested that sugars 



maj' be added if desired. 

 Reference: Faroy and Chavaillon (1915 p. 

 455). 



1867. Huntoon's Hormone Blood Agar 



Constituents: 



1. Water 1000.0 cc. 



2. Beef heart or steak (fresh). 500.0 g. 



3. Peptone (Bacto) 10.0 g. 



4. Agar 16.0 g. 



5. Salt 5.0 g. 



6. Whole egg (one) 



7. Blood, defibrinated 

 Preparation : 



(1) Chop 2 and then mix with 1, 3, 4, 

 5 and 6. Place in an enamel ware 

 vessel or a large coffee pot. 



(2) Heat over a free flame with constant 

 stirring until the red color of the 

 meat infusion changes to brown at a 

 temperature of about 68°C. Do not 

 go beyond this temperature. 



(3) Adjust to slightly alkaline to litmus 

 with N/1 NaOH and then add 1.0 

 cc. per liter of medium. 



(4) Cover the vessel and place in an 

 Arnold sterilizer or in a water bath 

 at 100° for one hour 



(5) Remove the vessel from the sterilizer 

 and separate with a glass rod the 

 firm clot, which has formed, from the 

 side of the vessel. 



(6) Return to the Arnold sterilizer and 

 heat at 100° for 1§ hours. 



(7) Remove the vessel and allow to 

 stand at room temperature for about 

 ten minutes in a slightly inclined 

 position. 



(8) Pipette off the fluid portion or 

 decant. If it is poured thru a fine 

 wire sieve, many of the fine pieces of 

 meat clot may be caught. (Avoid 

 filtering thru cheese cloth, cotton or 

 other adsorptive materials.) 



(9) Allow (8) to stand in tall cylinders 

 for 15 to 20 minutes until the fat 

 present has risen to the surface and 

 removed. The medium may be 

 further cleared by filtering thru 

 glass wool, asbestos wool, sedimenta- 

 tion or centrifugation. 



(10) Tube in 10.0 cc. lots. 



(11) Add a small amount (1.0 to 100.0 

 cc.) of defibrinated blood (just 

 enough to give a pinkish tinge to the 

 poured plate) to each tube of sterile 

 (10). 



SteriUzation : Sterilize (10) intermittently 

 in the steamer. 



Use: To cultivate highly parasitic organ- 

 isms. Author reported the medium es- 

 pecially adapted to the cultivation of the 

 meningococci. The medium is superior 

 to glucose ascitic agar. 



Variants: Huntoon reported better results 

 were obtained using laked blood instead 

 of defibrinated. 



Reference: Huntoon (1918 p. 171). 



1868. Torrey and Buckell's Ascitic Fluid 



Egg Agar 

 Constituents : 



1. Distilled water 1000.0 cc. 



2. Beef heart 500.0 g. 



3. Whole egg 1 



4. Peptone (Difco) 15.0 g. 



