590 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



(2) Dissolve 20.0 g. gelatin and 20.0 

 g. peptone in warmed (1). 



(3) Dissolve 10.0 g. of agar in 500.0 

 cc. of water and cool to below 

 50°C. 



(4) Mix (2) and (3). 



(5) Adjust the reaction. 



(6) Heat to coagulate the albumin 

 and clear the medium. 



(7) Readjust the reaction and reheat 

 if necessary. 



(8) Filter. 



(9) Sterilization not specified. 

 References: Frothingham (1895 p. 53), 



North (1909 p. 361), Tanner (1919 p. 51), 

 Dopter and Sacqu^pee (1921 p. 128), 

 Abbott (1921 p. 144), Harvey (1921-22 

 p. 71), Stitt (1923 p. 39), Park, Williams 

 and Krumwiede (1924 p. 118). 



1875. Krause's Gelatin Urea Agar 

 Constituents: 



1. Infusion agar (3.0%) 1000.0 cc. 



2. Infusion gelatin (20.0%).... 2000.0 cc. 



3. Urea (2.5%) 75.0 g. 



Preparation : 



(1) Prepare a 3.0% meat infusion agar. 



(2) Prepare a 20.0% meat infusion gelatin. 



(3) The NaCl content of (1) and (2) 

 should be about 0.7 to 0.8%. The 

 reaction is not adjusted. 



(4) Mix one part (1) with two parts (2). 



(5) Adjust the reaction to 0.27 to 0.3% 

 lactic acid. 



(6) Dissolve 2.5% urea in the least 

 amount of water and thoroly mix with 

 (5). 



(7) Distribute into sterile tubes. 



(8) Before use, melt sterile (7) and pour 

 into sterile plates. 



Sterilization: Steam (7) for 15 minutes in 

 flowing steam. 



Use: Detection of typhoid bacilli. Author 

 reported that typhoid colonies had a 

 nucleus surrounded by thread-like ap- 

 pendages, they were grey in color when 

 young and brown when old. 



Reference: Krause (1902 p. 94). 



1876. MacNeal and Kerr's Gelatin Agar 

 Constituents: 



1. Infusion broth 1000.0 cc. 



2. Agar 7.5 g. 



3. Gelatin 50.0 g. 



4. Blood serum (Sterile) 500.0 cc. 



Preparation : 



(1) Prepare usual infusion broth. 



(2) Dissolve 2 and 3 in (1) by heat and 

 tube. 



(3) Cool sterile (2) to 45°C. 



(4) Add 4. (i volume of 4 to (3)). 



(5) Inoculate while still a liquid. 



(6) Mix medium and solidify by immers- 

 ing in cold water. 



Sterilization: Sterilize (2). (Method not 



given). 

 Use: Culture medium for BaczV^us abortus. 



The authors reported a growth zone 5 mm. 



beneath the surface of the medium and 



extending down for 1.0 to 1.5 cm. 

 Reference: MacNeal and Kerr (1910 p. 



469). 



1877. Supplee's Nutrose Gelatin Agar 

 (Ayres and Johnson) 

 Constituents : 



1. Meat infusion 1000.0 cc. 



2. Gelatin 20.0 g. 



3. Agar lO.O g. 



4. Peptone 20.0 g. 



5. Nutrose 5.0 g. 



Preparation : 



(1) Prepare meat infusion. 



(2) Dissolve 2, 3, 4 and 5 in (1). 



(3) Adjust to neutral with phenol- 

 phthalein. 



Sterilization: Not specified. 



Use: Used especially in carrying stock 



cultures of streptococci and similar 



organisms. 

 Reference: Ayers and Johnson (1924 p. 



111). 



1878. Kinsella, Brown and Garcia's Nutrose 

 Gelatin Agar 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Beef 500.0 g. 



3. Peptone 10.0 g. 



4. NaCl 5.0 g. 



5. Agar 5.0 g. 



6. Gelatin 50.0 g. 



7. Nutrose 10.0 g. 



Preparation : 



(1) Mix finely chopped beef in water and 

 set in the ice chest for 24 hours. 



(2) Strain thru linen towel. 



(3) Add and dissolve 3 and 4 in (2). 



(4) Boil to clear and filter thru filter 

 paper. 



