694 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



(5) Add 1.0 cc. defibrinated sterile human 

 blood at 45°C., and 3.0 cc. 1.0% alco- 

 holic rosolic acid at 45 °C. 



(6) Prepare plates. 



(7) Keep two days before use. 

 Sterilization: Not specified. 



Use: Cultivation of colon-typhoid group. 

 Reference: Harvey (1921-22 p. 73). 



1885. Harvey's Saponin Blood Agar 



Constituents : 



1. Infusion agar. 



2. Saponin. 



3. Blood, defibrinated. 

 Preparation : 



(1) See variant (v) medium 1661 for 

 preparation of infusion agar. 



(2) Make a saturated solution of de- 

 fibrinated blood in 1.0% saponin. 



(3) Mix (1) and (2), amounts not given. 

 Sterilization: Not specified. 



Use: General culture mediiun. 

 Reference: Harvey (1921-22 p. 76). 



1886. Warden's Blood Veal Agar 



Constituents : 



1. Veal infusion 1000.0 cc. 



2. Agar 20.0 g. 



3. Blood, defibrinated rabbit.. 500.0 cc. 

 Preparation : 



(1) Prepare salt free veal infusion broth. 



(2) Dissolve agar in (1). 



(3) Neutralize to phenolphthalein. 



(4) Add defibrinated rabbit blood. 

 Sterilization: Not specified. 



Use: Cultivation of gonococci. 

 Reference: Warden (1915 p. 426). 



1887. Wherry and Ervin's Glycerol Blood 

 Agar 



Constituents : 



1. Beef infusion 1000.0 cc. 



2. Peptone (Witte's) 10.0 g. 



3. Na2HP04 5.0 g. 



4. Glycerol 60.0 g. 



5. Blood, defibrinated rabbit.. 100.0 cc. 

 Preparation : 



(1) Prepare lean beef infusion. 



(2) Dissolve2, 3, and4in(l). 



(3) Adjust to a reaction from -f-1.5 to 

 +2.0 to phenolphthalein. 



(4) Tube in 5.0 cc. lots. 



(5) Add to each sterile tube of (4) 0.5 cc. 

 of defibrinated rabbit blood. 

 Sterilization: Sterilize (4) in the autoclave. 

 Use: Cultivation of B. tuberculosis. 

 Reference: Wherry and Ervin (1918 p. 194). 



1888. Elser and Huntoon's Basal Blood 

 Infusion Agar 



Just before inoculation of tube of medium 

 1677, cover the slanted surface with several 

 drops of defibrinated human blood which 

 has been allowed to age for some time in 

 order to reduce to the minimum its initial 

 bactericidal properties. Authors reported 

 this medium especially adapted to the culti- 

 vation of freshly isolated cultures. 



1889. North's Gelatin Blood Agar (Kligler) 



Same as medium 1874 with the addition of 

 1.0 cc. of defibrinated rabbit blood per 

 10.0 cc. tube of North's gelatin agar. 

 Reference: Kligler (1915 p. 329). 



1890. Dieudonne's Alkaline Blood Agar 



(Harvey) 



Constituents : 



1. Infusion agar 140.0 cc. 



2. Blood, defibrinated ox 10.0 cc. 



3. NaOH (normal solution) 10.0 cc. 



Preparation : 



(1) Mix equal parts defibrinated ox blood 

 and normal NaOH solution (or 11.4% 

 Na2C03 may be used instead of 

 NaOH). 



(2) See variant (v) medium 1661 for 

 preparation of infusion agar. 



(3) Neutralize (2) to litmus. 



(4) Mix 3 parts sterile (1) at 45 °C. with 7 

 parts sterile (3) cooled to 45°C. 



(5) Distribute immediately into test 

 tubes or plates. Should be kept 

 freely open after tubing or plating, 

 under sterile sheets of paper, for 48 

 hours at 37°C., or under a bell jar 

 containing carbon dioxide gas for 30 

 to 60 minutes. 



Sterilization: Sterilize (1) at 100°C. on 



each of 3 successive days. 

 Use: Isolation of V. cholerae. 

 Variants: Harvey gave the following 



variants. 



(a) (1) Mix equal parts normal NaOH 

 and defibrinated ox blood. 



