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CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



(5) Take up a drop of the blood which 

 exudes in a sterile platinum loop. 



(6) Smear on the surface of an agar 

 slope. (Serum may be used in the 

 same way.) 



(7) Cover the test tube with an India- 

 rubber cap. 



(8) Test sterility before use by incuba- 

 tion 48 hours. 



(d) Harvey also prepared a medium as 

 follows : 



(1) Add 0.25 cc. blood to 5.0 cc. melted 

 agar infusion in a test tube. (See 

 variant (v) medium 1661 for prep- 

 aration.) 



(2) Boil one minute. 



(3) Slope. (The precipitate settles to 

 the bottom.) 



(e) Harvey prepared a similar medium as 

 follows: 



(1) Add directly, blood drawn off from 

 a vein or from the heart with a 

 sterile syringe to melted infusion 

 agar in a test tube at 45°C. (See 

 variant (v) medium 1661.) 



(2) Roll the test tube between the 

 palms to mix. 



(3) Slope. 



(4) Test sterility by incubation 48 

 hours. 



References: Grassberger (1897 p. 462), 

 Waksman (1919 p. 210), Twort and Twort 

 (1921 p. 88), Harvey (1921-22 p. 72). 



1904. Ruediger's Blood Agar 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Veal, minced 500.0 g. 



3. Peptone, Bacto 10.0 g. 



4. Bacto agar 15.0 g. 



5. NaCl 5.0 g. 



6. Blood, human 100.0 cc. 



Preparation : 



(1) Boil 2 in 1 for one hour. 



(2) Cool and strain thru cheese cloth. 



(3) Neutralize to phenolphthalein with 

 N/1 NaOH. 



(4) Dissolve 3, 4 and 5 in (3). 



(5) Tube in 10.0 cc. lots. 



(6) Heat human blood to 56°C. for 30 

 minutes. 



(7) Add 1.0 cc. of (6) to 10.0 cc. of sterile 

 (5). 



(8) Slant and solidify. 



(9) Stopper tubes air tight with sterile 

 cork stoppers. 



Sterilization: Sterilize in the autoclave. 



Use: Cultivation of gonococci. The 

 author reported that the addition of 1.0% 

 glycerol or 1.0% glucose inhibited growth 

 just as did the absence of peptone. 



Reference: Ruediger (1919 p. 377). 



1905. Dieudonne's Alkaline Blood Agar 



(Tanner) 

 Constituents : 



1. Infusion agar 700.0 cc. 



2. Blood, beef 150.0 cc. 



3. KOH (N/1) 150.0 cc. 



Preparation : 



(1) Mix equal volumes of beef blood and 

 normal KOH solution. 



(2) Steam for 30 minutes. 



(3) Mix 3 volumes of (2) with 7 volumes of 

 neutral (to litmus) infusion agar. 



(4) Pour into plates. 



(5) Dry the plates at 60°C. for 30 minutes, 

 and at room temperatures for 24 

 hours before use. 



Sterilization: Not specified. 



Use: Cultivation of Microspira cholerae. 



Reference: Tanner (1919 p. 70). 



1906. Sherwood and Downs' Glucose Blood 



Agar 



Constituents : 



1. Infusion agar 1000.0 cc. 



2. Glucose 20.0 g. 



3. Blood (whole) 50.0 cc. 



Preparation : 



(1) Prepare meat infusion agar with a re- 

 action of +0.2% to phenolphthalein. 



(2) Add 20.0 g. glucose and 50.0 cc. of 

 whole blood to 1000.0 cc. of (1). 



Sterilization: Not specified. 



Use: To determine hemolytic ability of 



streptococci. 

 Reference: Sherwood and Downs (1919 p. 



135). 



1907. Hall's Testicular Infusion Blood Agar 



(Stitt) 

 Constituents : 



1. Distilled water 1000.0 cc. 



2. Testicles, beef 500.0 g. 



3. Peptone (2.0%) 20.0 g. 



4. Glucose (0.5%) 5.0 g. 



5. NaH2P04 (0.2 to 



0.3%) 2.0 to 3.0 g. 



