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CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Use: Cultivation of meningococci, pseudo- 

 meningococci and gonococci. Authors 

 reported that the medium was especially- 

 suited to freshly isolated cultures. 

 Added nutrients: The author added 1.0% 

 of one of the following: 



glucose sucrose 



galactose mannitol 



levulose inulin 



lactose dextrin 



maltose dulcitol 



Variants : 



(a) Plotz, Olitsky and Baehr used the 

 following medium to study fermenta- 

 tion by organisms causing typhus 

 exanthematicus: 



(1) Prepare 3.0% agar. 



(2) Add 2.0% of one of the added nu- 

 trients to (1). 



(3) Add § volume of ascitic fluid (spe- 

 cific gravity to be 1013 or over). 



(4) Reaction of medium to be between 

 1.0 and 1.5%. 



(5) Tinge with Kahlbaum's litmus. 



(6) Method of sterilization not given. 

 The added nutrients employed 

 were: 



glucose arabinose 



lactose inulin 



sucrose raffinose 



maltose galactose 



mannitol dextrin 



(b) Harvey prepared a medium as 

 follows: 



(1) Mix one part nutrose with 15 parts 

 ascitic fluid and 35 parts distilled 

 water. 



(2) Raise slowly to boiling tempera- 

 ture with frequent shaking to pre- 

 vent burning. 



(3) Boil or steam 30 minutes. 



(4) Clarify by the addition of egg and 

 filter thru thick filter paper. 



(5) Add one part (4) at 45 °C. to two 

 parts melted infusion agar cooled 

 to 45°C. (see variant (v) medium 

 1661 for preparation). 



(6) Steam 30 minutes. 



(7) Distribute into test tubes. 



(8) Sterilize. 



(c) Harvey cultivated meningococci on a 

 mixture of one part ascitic fluid at 

 45°C. with three parts melted infu- 

 sion agar, (see medium 1661, variant 



(v) for preparation) containing 1.0% 

 glucose at 45°C. Reaction of the 

 agar to be faintly alkaline to litmus, 

 (d) Harvey cultivated meningococci on a 

 mixture of seven parts sterile ascitic 

 fluid at 45 °C., three parts of a sterile 

 standard litmus solution at 45 °C., two 

 parts of a sterile 10.0% solution of 

 any desired carbohydrate at 45 °C., 

 0.15 part of a normal NaOH solution 

 at 45 °C., and 21 parts of melted infu- 

 sion agar at 45°C. (See variant (v) 

 medium 1661 for preparation.) The 

 litmus solution was prepared as 

 follows: 



(1) Place in a well stoppered glass 

 bottle, solid commercial litmus 1; 

 95.0% alcohol 6. 



(2) Shake one daily for seven days. 



(3) Reject the alcohol. 



(4) Add fresh 95.0% alcohol. 



(5) Shake well once daily. 



(6) Repeat the addition of fresh alco- 

 hol until the alcohol becomes only 

 lightly tinged with violet on shak- 

 ing up with the litmus. 



(7) Reject the alcohol. 



(8) Dry the alcohol insoluble residue. 



(9) Make a saturated solution of the 

 dried residue in distilled water. 



(10) Filter. 



(11) Dilute a portion of this saturated 

 solution with distilled water until 

 its tint is a pure blue. 



(12) Add to this pure blue solution very 

 dilute sulphuric acid till the blue 

 color is turned to wine red. 



(13) Add to this wine red solution the 

 saturated solution obtained from 

 the alcohol insoluble residue until 

 the blue color returns. 



(14) Use as a sensitive litmus solution. 

 Sterile 10.0% sugar at 45°C., 2; 

 sterile N/1 sodium hydroxide at 

 45°C., 0.15; melted nutrient agar 

 at 45°C., 21. 



(e) Harvey cultivated B. acnes on a 

 medium composed of 2 parts sterile 

 oleic acid, 20 parts sterile ascitic 

 fluid, 0.8 part of a sterile 1.0% 

 neutral red and 100 parts of sterile 

 infusion agar (see medium 1661, 

 variant (v) for preparation). 



(f) Torrey and Buckell used the follow- 



