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CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



(11) Render slightly alkaline to litmus 

 while hot if necessary. 



(12) Filter thru glass wool into a sterile 

 vessel. 



(13) To 130.0 cc. of litmus solution 

 (Kubel and Tiemann) add 15.0 g. 

 c.p. lactose. 



(14) Boil (13) for 10 minutes. 



(15) Mix (14) and (12) while hot. 



(16) Render slightly alkaline to litmus 

 paper if necessary. 



(17) To (16) add 2.0 cc. of hot sterile 

 solution of 10.0% sodium hydrate 

 in distilled water, and 10.0 cc. of 

 fresh solution of Hochst's crystal 

 violet (0.1 g. to 100.0 cc. of sterile 

 water). 



(18) Pour in sterile Petri dishes. Glass 

 covers of Petri dishes may be dis- 

 carded and use porous clay covers. 



(d) Reitz used the following medium to 

 study the bacteriology of butter and 

 butter examination: 



(1) Chop 3 pounds of horse meat to 

 small pieces. 



(2) Pour 2 liters of water over (1) and 

 allow to stand until the next day 

 (in ice box). 



(3) Press the water from the meat. 



(4) Add 2.0% Witte peptone, 2.0% 

 nutrose and 0.5% NaCl to the meat 

 water and boil an hour. 



(5) Filter thru a linen towel and add 

 3.0% agar. 



(6) Boil in the steamer for 3 hours and 

 filter thru linen in the steamer. 



(7) Prepare 300.0 cc. of a 15.0% Kahl- 

 baum's litmus solution. 



(8) Add 30.0 g. lactose to (7) and boil 

 for 15 minutes. 



(9) Mix hot (8) and hot (6). 



(10) Add 10.0% soda solution until the 

 reaction is slightly alkaline to 

 phenolphthalein. 



(11) To (10) add 6.0 cc. of a sterile warm 

 10.0% soda solution and 20.0 cc. 

 of a fresh solution of 0.1 g. crystal 

 violet (Hochst c.p.) in 100.0 g. 

 sterile distilled water. 



(12) Distribute in 200.0 cc. lots in 

 sterile Erlenmeyer flasks. 



(13) Final sterilization not specified, 

 (e) Schmitz used the following medium 



as an elective medium for typhoid 

 bacilli: 



(1) Obtain 7 or 8 liters of blood from 

 the slaughter house. 



(2) Pour off the serum from the clot. 



(3) Add a double amount of water to 

 the clot. 



(4) Boil the mixture. Stir constantly 

 but take care not to break up the 

 clot too much or filtration will be 

 too diflficult. Boil for 5 to 10 

 minutes. 



(5) Filter thru a towel, then thru cot- 

 ton or glass wool. 



(6) Add peptone nutrose, in the usual 

 amount (exact amount not speci- 

 fied) and 3.0% agar. 



(7) When the agar has been completely 

 dissolved, add the serum that had 

 been previously poured from the 

 blood and that had been allowed 

 to stand undisturbed. 



(8) Boil for a few minutes. 



(9) Place in a flask with a patented 

 stopper and heat for one hour in 

 streaming steam. 



(10) Allow to cool slowly so that the 

 sediment and turbidity may settle 

 to the bottom. Use only the top 

 clear medium. 



(11) If caffeine is desired in the medium 

 prepare a solution of caffeine in 

 water so that 1.0 cc. of solution 

 contains 0.1 g. caffeine. Sterilize 

 and add 6.0 cc. of this solution to 

 100.0 cc. of the agar. 



(f) Roddy. 



(1) Mix 750.0 g. of finely minced beef 

 with 1000.0 cc. of water. 



(2) Place in a shallow dish in the ice 

 ice box over night. 



(3) Skim off the fat. 



(4) Boil for one hour. 



(5) Filter. 



(6) Bring this volume to 1000.0 cc. 



(7) Add 10,0 g. Witte's peptone, 10.0 g. 

 nutrose and 5.0 g. CaCl2 to (6). 



(8) Boil. 



(9) Add 30.0 g. agar and boil until 

 solution is complete. 



(10) Make faintly alkaline to litmus. 



(11) Autoclave for one hour. 



(12) Filter thru paper. 



