CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



621 



Sterilization: Sterilize (9) in streaming 

 steam for 15 minutes on each of 2 suc- 

 cessive days. 



Use: General culture medium. 



Reference: Roddy (1917 p. 44). 



1941. Schmitz's Nutrose Blood Clot 

 Infusion Agar 



1000.0 cc. 

 500.0 g. 



Constituents : 



1. Water 



2. Blood clot 



3. Peptone 



4. Nutrose 



5. Agar (3.0%) 30.0 g. 



Preparation : 



(1) Obtain 7 or 8 liters of blood from the 

 slaughter house. 



(2) Pour off the serum from the clot. 



(3) Add a double amount of water to 

 the clot. 



(4) Boil the mixture. Stir constantly 

 but take care not to break up the 

 clot too much or filtration will be 

 too difficult. Boil for 5 to 10 

 minutes. 



(5) Filter thru a towel, then thru cot- 

 ton or glass wool. 



(6) Add peptone nutrose, in the usual 

 amount (exact amount not specified) 

 and 3.0% agar. 



(7) When the agar has been completely 

 dissolved, add the serum that had 

 been previously poured from the 

 blood and that has been allowed to 

 stand undisturbed. 



(8) Boil for a few minutes. 



(9) Place in a flask with a patented 

 stopper and heat for one hour in 

 streaming steam. 



(10) Allow to cool slowly so that the 

 sediment and turbidity may settle 

 to the bottom. Use only the top 

 clear medium. 

 Sterilization: Not specified. 

 Use: Selective medium for typhoid bacilli. 

 Variants: The author gave the following 

 variants: 



(a) If caffeine is desired in the medium 

 prepare a solution of caffeine in water 

 so that 1.0 cc. of solution contains 

 0.1 g. caffeine. Sterilize. Add 

 6.0 cc. of this solution to 100.0 cc. of 

 the agar. 



(b) (1) Mix 15 parts lactose and 1 part 

 Congo red. 



(2) To 100.0 CC. of the sterile melted 

 agar, add 1.5 g. (1). 



(3) Pour the red medium into sterile 

 Petri dishes. 



(4) If caffeine is desired in the me- 

 dium prepare a solution of caf- 

 feine in water so that 1.0 cc. of 

 solution contains 0.1 g. caffeine. 

 Sterilize and add 6.0 cc. of this 

 solution to 100.0 cc. of the agar. 



Reference: Schmitz (1915 pp. 307, 315). 



1942. Harvey's Milk Agar 



Constituents : 



1. Infusion agar. 



2. Milk. 



Preparation: (1) Add 3.0 cc. of sterilized 

 milk to a tube of melted infusion agar (see 

 variant (v) medium 1661 for prepara- 

 tion). 



Sterilization: Not specified. 



Use: General culture medium. 



Variants: Harvey gave the following 

 variants : 



(a) Use litmus milk instead of plain 

 milk. 



(b) Mix equal parts of sterilized milk at 

 45°C. and melted infusion agar at 

 45°C. (See variant (v) medium 1661 

 for preparation). 



Reference: Harvey (1921-22 p. 95). 



1943. Lubenau's Caffeine Whey Agar 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Beef 500.0 g. 



3. NaCl 100 g- 



4. Peptone 60.0 g. 



5. Agar 40.0 to 60.0 g. 



6. Litmus whey 900.0 cc. 



7. Caffeine 6%, solution 



Preparation : 



(1) Boil 500.0 g. of finely chopped lean 

 beef with one liter distilled water for 

 30 minutes. 



(2) Filter and make up to one liter. 



(3) Add 3, 4, and 5 to (2) and dissolve by 

 boiling in a salt water bath. 



(4) Neutralize to litmus. 



(5) Boil and filter. 



(6) Add 900.0 cc. of a sterile litmus whey, 



