622 CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



(preparation not given) to hot sterile Variants : Schloffer reported that urine may 



n\ M,v +K . 1 ^ 11 . , ^® collected in sterile flasks and then 



S T^a no n "^ ' f T -^ "l"L ^'^''"^ *« ^' °^ 80=C. to sterilize. 



(8) Add 110.0 cc. of a sterile 6.0% caf- References: Schloffer (1893 p. 657), Ghon- 

 feme solution to (7). Mix well. Schlagenhaufer (1893 p. 619) 



(9) Pour in plates. ^' 

 Sterilization: Method of sterilization of (5) ^^'^^- dicker's Glycerol Sputum Agar 

 ^"«*gi^en. Constituents: 



Use : Detection of typhoid bacteria. 1. infusion agar (2.0%) 200 cc 



Authors reported that typhoid colonies 2. Sputum inn n 



were colorless. 3. Glycerol :.:::::;::::::: so cc' 



Reference: Lubenau (1907 p. 248). Preparation: " " ' 



1944. Harvey's Whey Infusion Agar ^^^ Prepare neutral 2.0% infusion agar. 



Constituents: ^'^ "^ZH Tu ''T"' ^'^ "?^ ' P'^* 



1. Infusion agar. ll^t *"^^^^^^°"« °^ bronchitus 



^ ^- ^'^7- (3) Add 2.0% glycerol. 



m MiTlOOO n f f . -H • u ^^^ ^°"^ ''^ ^^°' "^^'^ *^«^°ly ^^d allow to 



(1) Mix 1000.0 cc. of fresh milk with solidify. 



(9^ K^ ''■ fJr'oi^^^- Sterilization: 'sterilize tuberculous or bron- 



3 Fit ''^'^''^ ^^""^^ ^°'" ^"^ ^''''' "'^ ^^^^ °^ 3 suc- 



Jzi AT i^^\i. fiu . . , ,. cessive days in the steamer. Method of 



(4) Make the filtrate neutral to litmus sterilization of (1) not given 



with sodium hydroxide. Use: Cultivation of tubercle bacilli. 



(5 Steam 2 hours. Reference: Ficker (1900 p. 508). 

 (b) Histimate and adjust the reaction. 



(7) Filter. 1947. Szaboky's Sputum Lung Infusion Agar 



(8) Mix one part sterile (7) with two parts Constituents : 



sterile infusion agar (see variant (v) 1. Water .. 200 cc 



medium 1661 for preparation). 2. Lung infusion agar.'.' .' ^ ' ^ ' 50 cc' 



Sterilization: Sterilize (7) at 100°C. 3. Sputum 50 l 



Use: Cultivation of gonococci. 4 NaCl 5 



Reference: Harvey (1921-22 p. 95). 5^ Glucose '.[[[[]][][[[[[[][][] o.5 g.' 



1945. Schloffer's Urine Infusion Agar ^ Glycerol 12.0 g. 



^ 7. Peptone 10? 



Constituents: Preparation: ^• 



1. Infusion agar (2.0%) 200.0 cc. (1) See medium 1746 for preparation of 



^2- Urine 100.0 cc. lung infusion agar. 



Preparation: (2) Boil slowly for one hour. 



(1) Prepare a 2.0% infusion agar. (3) Filter while hot 



(2) Obtam urine under aseptic con- (4) Adjust to neutral or |% acid. 



/o^ aI^^^^^' ., Sterilization : Sterilize on 3 successive days 



(3) Mix two parts sterile agar with one in streaming steam 



r^>??'!'l'f'™''- ., ^'^' Cultivation of tubercle bacilli. 



(4) Distribute mto sterile plates, tubes Author reported that growth appeared in 

 or flasks two days with neutral or |% acid medium. 



Sterihzation: Method of sterilization of in- Reference: Szaboky (1906 p. 657) 

 fusion agar not given. 



Use: Cultivation of diphtheria bacilli. 1^48. Costa and Boyer's Gum Infusion Agar 



Author reported that in pure culture Constituents: 



colonies were grayish-white to white, 1. Water 



quite luxuriant and rather dry. Ghon- 2. Beef 500 g 



Schlagenhaufer used a similar medium for 3. NaCl 5 e 



the isolation of gonococci. 4' Peptone. ..'.'.'. ...'..'.'.'. ' ." [ '. [ ] ] [ 2o!o g. 



