CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



625 



1953. Lipschiitz's Egg Albumin Agar 



Same as medium 953 but the bouillon is 

 solidified by the addition of 1.0% agar. 



1954. Krumwiede, Pratt and Grund's Egg 



Albumin Agar 

 Constituents: 



1. Water 150.0 cc. 



2. Egg albumin 150.0 cc. 



3. NaoCOs 3.6 g. 



4. Nutrient agar (3.0%) 700.0 cc. 



Preparation : 



(1) Mi.x equal parts of 1 and 2 and add 3. 

 (May be filtered thru thin layer of 

 cotton to remove any thick parts of 

 egg.) 



(2) Steam (1) in Arnold sterilizer for 

 20 minutes. 



(3) Prepare 3.0% nutrient agar neutral 

 to litmus. 



(4) IVIix (2) and (3) while (3) is boiling hot. 



(5) Pour medium thick plates, allow them 

 to stand open for 20 to 30 minutes. 



Sterilization: Method not given. 



Use: Isolation of cholera vibrio. Authors 

 reported that by transmitted light 

 cholera colonies appeared deep in the 

 agar with hazy appearance. Medium in- 

 ferior to a whole egg medium. 



Variants: Sacquepee (Bezangon) gave the 

 following method of preparation: 



(1) Add 200.0 cc. of sterile water to the 

 whites of two eggs. 



(2) Heat to 50°C. 



(3) Make alkaline by adding 0.5 cc. of 

 10.0% soda solution for each 100.0 cc. 

 of egg solution. 



(4) Sterilize in the autoclave. 



(5) Prepare 3.0% agar neutral to litmus. 



(6) Add 9.0 cc. of 10.0% soda solution to 

 a liter of (5). 



(7) Add 200.0 cc. of (4) to 1000.0 cc. of (6). 



(8) Sterilize in the autoclave at 115°C. 

 References: Krumwiede, Pratt and Grund 



(1912 p. 137), Bezan?on (1920 p. 121). 



1955. Oberstadt's Egg Albumin Agar 



Same as medium 952 but the bouillon is 

 solidified by the addition of agar. 



1956. Besredka and Jupille's Egg Agar 



(Besson) 

 Constituents : 



1. Nutrient agar 



2. Bouillon 500.0 cc. 



3. Egg albumin (10.0% solution) 400.0 cc, 



4. Egg yolk (10.0% solution). . . 100.0 cc. 

 Preparation : 



(1) Mi.x 5 parts bouillon with 4 parts of a 

 10.0% solution of egg white in sterile 

 water and one part of a 10.0% egg 

 yolk emulsion in sterile water. If the 

 eggs are not obtained under aseptic 

 conditions, the solutions may be 

 sterilized by heating at 55 °C. 



(2) Add 4.0 cc. of (1) to the surface of 

 solidified agar and allow to stand for 

 24 hours. 



Sterilization: Not specified. 

 Use: General culture medium. 

 Reference: Besson (1920 p. 55). 



1957. Scales' Whole Egg Agar 



Constituents : 



1. Nutrient agar (1.5%) 70.0 cc. 



2. Whole egg 20.0 cc. 



Preparation: 



(1) Strain fresh eggs thru four thick- 

 nesses of cheese cloth. 



(2) Prepare 1.5% nutrient agar. 



(3) Thoroly mix 20.0 cc. of (1) with 

 70.0 cc. of melted (2). 



Sterilization: Sterilize at 12 pounds for 



15 minutes. 

 Use: To study variation in morphology of 



B. coll. 

 Variants: The author added 1.0% glucose. 

 Reference: Scales (1921 p. 595). 



1958. Stitt's Glycerol Egg Agar 



Constituents: 



1. Glycerol agar. 



2. Egg. 

 Preparation : 



(1) Take the yolk and white of one egg 

 and mix thoroly with an equal amount 

 of glycerol agar, at a temperature of 

 45 to 55 °C. 



(2) Tube. 



(3) Inspissate in a rice cooker as for 

 serum tubes. 



(4) Since egg medium has a tendency to 

 be dry it is well to add 1.0 cc. glycerin 

 bouillon to each tube before steri- 

 lization. 



Sterilization: Sterilize in the same manner 



as for blood serum. 

 Use: Cultivation of tubercle bacilli. 

 Reference: Stitt (1923 p. 38). 



