626 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



1959. Robertson's Alkaline Egg Agar (Park, 



Williams and Krumwiede) 

 Constituents: 



1. Water 500.0 cc. 



2. Egg 



3. NaOH (normal solution) .... 6.0 cc. 



4. Nutrient agar 

 Preparation : 



(1) Mix the yolk of one egg, the whites 

 of two eggs, 500.0 cc. of water and 

 6.0 cc. of N/1 NaOH. 



(2) Mix one part (1) with five parts agar. 

 Sterilization: Not specified. 



Use: Cultivation of cholera organisms. 

 Reference : Park, Williams and Krimiwiede 

 (1924 p. 126). 



1960. Cantani jun's Sperm Agar 

 Constituents : 



1. Nutrient agar. 



2. Sperm. 

 Preparation : 



(1) Remove the testicles from a bull 

 under aseptic conditions. 



(2) Extract the sperm by pressing the 

 Funiculus sperm aliens between two 

 fingers and remove the sperm, thus 

 pressed out with a sterile platinum 

 loop. 



(3) Streak the sperm on sterile agar 

 slants. 



(4) Incubate to prove sterility. 

 Sterilization: Not specified. 



Use: Cultivation of influenza bacilli, tuber- 

 cle bacilli and others. 



Variants: A similar medium was prepared 

 as follows: 



(1) Prepare nutrient agar. 



(2) Free testicular material of its capsule. 



(3) Wash the outer surface with alcohol 

 and ether. 



(4) When dry cut cross sections with a 

 sterile knife. 



(5) Remove some of the liquid from the 

 cross sections by means of a strong 

 platinum loop. 



(6) Streak the liquid on sterile solidified 

 agar slants. 



(7) Incubate 10 hours to prove sterility. 

 References: Cantani jun (1897 p. 601). 



1961. Pettersson's Brain Ascitic Fluid Agar 

 Constituents : 



1. Nutrient agar. 



2. Ascitic fluid. 



3. Brain. 



Preparation: 



(1) Remove the brain under aseptic con- 

 ditions from a dead new-born human 

 foetus, and place in a sterile covered 

 jar. 



(2) Add sterile ascitic fluid to (1) and 

 shake the mixture for an hour in a 

 shaking machine. 



(3) Place in the ice box and allow to stand 

 for several hours. 



(4) Mix the clear opalescent fluid with 

 an equal amount of 3.0% agar. 



Sterilization: Not specified. 



Use: Cultivation of gonococci. The use 



of glucose agar increases the vigor of 



growth. 

 Reference: Pettersson (1920 p. 1385). 



1962. Smith and Taylor's Fetus Agar 

 Constituents : 



1. Nutrient agar. 



2. Fetus tissue. 

 Preparation : 



(1) Prepare nutrient agar. 



(2) Slant. The tube should contain a 

 small quantity of water of condensa- 

 tion or add a little bouillon. 



(3) Add to each slant \ g. of tissue or an 

 equivalent amount of stomach or 

 other fluid or intestinal contents of 

 the fetus. 



(4) Seal the tubes with sealing wax. 

 Sterilization: Not specified. 



Use: Isolation of Vibrio felus. 

 Reference: Smith and Taylor (1919 p. 301). 



1963. Noguchi's Ascitic Fluid Tissue Agar 

 Constituents : 



1. Nutrient agar (2.0%) 200.0 cc. 



2. Ascitic fluid 100.0 cc. 



3. Tissue (rabbit testicle or 

 kidney) 



Preparation : 



(1) Prepare 2.0% nutrient agar. 



(2) Adjust (1) to slightly alkaline. 



(3) Place small pieces of fresh tissue 

 (preferably rabbit testicle or kidney, 

 but human placenta, sheep testicle or 

 other sterile organs will suffice) into 

 a tube measuring 2 by 20 cm. 



(4) Mix 2 parts melted (2) with one part 

 ascitic (or hydrocele) fluid and add 

 15.0 cc. to each tube of (3). 



(5) After solidification add a layer of 



sterile paraffin to prevent evap- 

 oration. 



