632 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



1977. Mandelbaum's Lactose Blood Agar 



Constituents : 



1. Nutrient agar. 



2. Lactose. 



3. Rosolic acid. 



4. Blood, defibrinated human. 

 Preparation : 



(1) To a tube containing 8-10.0 cc. nu- 

 trient agar is added 0.3 g. lactose. 



(2) Heat to 100 °C. 



(3) Cool to 50°C. 



(4) Add 0.3 cc. 1.0% alcoholic rosolic 

 acid solution. 



(5) Add 1.0 cc. defibrinated sterile human 

 blood (Probably animal blood may 

 be used). 



(6) Mix and pour into Petri dishes. 



(7) Stand 24 hours, or dry | hour at 37°C. 

 Sterilization: Not specified. 



Use: Isolation of colon typhoid group. 

 Reference: Mandelbaum (1912 p. 306). 



1978. Thompson's Glucose Plasma Agar 



Constituents : 



1. Nutrient agar (2.5%) 1000.0 cc. 



2. NaCl 9.0 g. 



3. CaCh 0.25 g. 



4. KCl 0.42 g. 



5. Glucose (2.5%) 25.0 g. 



6. Plasma (human) 

 Preparation : 



(1) Prepare nutrient agar (2.5%) in the 

 ordinary way using 1.0% Witte pep- 

 tone (Omit the NaCl). 



(2) Adjust to +6 acid. 



(3) Add 2, 3, 4 and 5 to (1). 



(4) Tube in 4.0 cc lots. 



(5) Melt sterile (4) in boiling water and 

 cool to 50°C. Then add 1.0 cc. of 

 human plasma to each tube. Mix and 

 slant. 



Sterilization: Method of sterilization of (4) 



not given. 

 Use: Cultivation of gonococci. 

 Reference: Thompson (1917 p. 869). 



1979. Avery's Oleate Blood Agar (Stitt) 



Constituents 



1. Nutrient agar 95.0 cc. 



2. Sodium oleate (2.0% soln.). . . 5.0 cc. 



3. Blood, defibrinated or citrated 

 Preparation : 



1 Prepare nutrient agar. 



(2) Adjust (1) to pH = 7.2 to 7.5. 



(3) To 95.0 cc. of (2), add 5.0 cc. of a2.2% 

 solution of neutral sodium oleate. 



(4) Add defibrinated or citrated blood to 

 (3) while it is still hot (temperature 

 or amount of blood not specified). 



Sterilization: Not specified. 



Use: Cultivation of Pfeiffer's bacilli. 



Reference: Stitt (1923 p. 43). 



1980. Esch's Ascitic Fluid Blood Agar 



Constituents : 



1. Nutrient agar 60.0 cc. 



2. Blood, defibrinated sheep 20.0 cc. 



3. Ascitic fluid 10.0 cc. 



4. Maltose 1.0 g. 



5. Bouillon 3.0 cc. 



Preparation : 



(1) Prepare nutrient agar containing 

 1.0% Witte's peptone. 



(2) Prepare bouillon. 



(3) Mix 60.0 cc. of (1) that has been 

 cooled to 50°C. with 20.0 cc. sterile 

 defibrinated sheep blood, 10.0 cc. 

 ascitic fluid and 1.0 g. of maltose dis- 

 solved in 3.0 cc. of bouillon. 



(4) Pour into sterile Petri dishes. 

 Sterilization: Not specified. 



Use: Cultivation and isolation of meningo- 

 cocci. Author reported that typical 

 colonies developed after 8 hours. 



References: Esch (1909 p. 153), Klimmer 

 (1923 p. 225). 



1981. Listen's Trypsinized Casein Blood 

 Agar 



Constituents : 



1. Distilled water 100.0 cc. 



2. Blood 20.0 cc. 



3. Trypsinized casein 100.0 cc. 



4. Agar (3.0%) 400.0 cc. 



Preparation : 



(1) A 200.0 cc. capacity flask, containing 

 a few glass beads and 100.0 cc. dis- 

 tilled water, is sterilized and kept 

 ready. 



(2) 20.0 cc. of human (or rabbit) blood, 

 removed with due precautions to 

 avoid contamination, are added to 

 this sterile distilled water. The 

 flask is then at once vigorously 

 shaken to prevent the formation of 

 large fibrin masses and the blood 

 soon lakes. 



(3) 5.0 cc. of an alcoholic pancreatic ex- 

 tract are then added to the flask. 



