CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



641 



to each tube, (strength of hemo- 

 globin solution not given). 



(3) Mix the tubes without shaking. 



(4) Cool in a slanting position. 

 Sterilization: Not specified. 

 Use: General culture medium. 

 Reference: Tanner (1919 p. 69). 



2001. Kabeshima's Hemoglobin Extract 

 Agar 



Constituents: 



1. Nutrient agar (3.07o) 1000-0 cc 



2. Hemoglobin extract 50.0 g. 



3. Saline solution 1000.0 cc. 



Preparation : 



(1) Add 0.1 cc. of an 18.0% NajCOa solu- 

 tion to 1000.0 cc. of neutrient neutral 

 agar. 



(2) Boil 10 minutes. 



(3) Cool to about 15°C. and add 1000.0 cc. 

 of hemoglobin extract prepared by 

 dissolving 50.0 g. of Shinoda's hemo- 

 globin extract in 1000.0 cc. of 0.9% 

 salt solution. 



(4) Distribute in petri dishes and remove 

 the bubbles by flaming with a Bunsen 

 Burner. 



(5) When cool remove the covers and 

 place in the incubator for 30 minutes. 



(6) Use when dry. 

 Sterilization: Not specified. 



Use: Cultivation of cholera vibrio. 

 Reference: Kabeshima (1916 #225) taken 

 from (1917 p. 395). 



2002. Finger, Ghon and Schlagenhaufer's 

 Dialyzed Serum Agar 



Constituents : 



1. Nutrient agar. 



2. Blood Serum (Dialyzed). 

 Preparation : 



(1) Place sterile human blood serum in 

 sterile parchment sack. 



(2) Suspend the blood serum in the sack 

 in a sterile cylinder containing sterile 

 water. 



(3) Change the water every 48 hours and 

 continue the dialysis until the water 

 is colored by the albumin that 

 dialyzes thru the parchment. 



(4) Mix the dialyzed blood serum with 

 agar in the same manner as in the 

 preparation of blood serum agar. 



Sterilization: Method not given. 



Use: Cultivation of gonococci. Authors 

 reported that growth was equally as good 

 in non-dialyzed serum medium. 



Reference: Finger, Ghon and Schlagen- 

 haufer (1894 p. 14). 



2003. Miiller's Serum Agar 



Constituents: 



1. Nutrient agar. 



2. Serum, goat. 

 Preparation : 



(1) Prepare nutrient agar. 



(2) Melt (1) and cool to about 60°C. 



(3) Mix equal parts of (2) and goat serum 

 that has been obtained under aseptic 

 conditions and heated to 60 °C. 



(4) Pour into sterile plates or sterile 

 tubes. 



Sterilization: Method of sterilization of 

 agar not given. 



Use : Cultivation of fowl diphtheria bacilli. 

 Similar media were used for the cultiva- 

 tion of various organisms by a number of 

 investigators. 



Variants : 



(a) Miihlens and Hartmann cultivated 

 Spirochaeta dentium and Bacillus 

 fusiformis on a medium prepared as 

 follows: 



(1) Prepare nutrient agar with a 

 slightly alkaline or neutral re- 

 action. 



(2) Boil half filled tubes of (1) for 

 about 30 minutes in a water bath to 

 free the agar of oxygen. 



(3) Heat horse serum for about 30 

 minutes in a water bath at a tem- 

 perature from 58 to 60°C. 



(4) Cool (2) and (3) quickly to 45°C. 



(5) Mix two parts of the agar with one 

 part of the serum. 



(6) Inoculate while the medium is still 

 in a liquid state. 



(7) Solidify by placing the tubes in 

 cold water. 



(b) Carrel mixed serum with one-fifth 

 its volume of 2.0% agar and used the 

 medium for the cultivation of tissue. 



(c) Besson prepared the medium as 

 follows: 



(1) Melt tubes of sterile nutrient agar. 



(2) Cool to 45° to 50°C. 



