CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



649 



(b) Stitt cultivated B. bulgaricus on a 



medium prepared by adding 2.0 or 



3.0 cc. of plain or litmus milk to a 



tube of melted nutrient agar. 



References: Hastings (1903 p. 384), Miiller 



(1906 p. 521), Tanner (1919), Klimmer 



(1923 p. 203), Stitt (1923 p. 42). 



2023. Valleti's Whey Agar 

 Constituents : 



1. Nutrient agar. 



2. Whey. 

 Preparation: 



(1) Prepare nutrient agar. 



(2) Prepare serum from cow's milk by 

 boiling milk with a few drops of 

 acetic acid. 



(3) Add 2.0 cc. of milk serum to agar 

 (amount not given). 



Sterilization: Not specified. 



Use: Cultivation of tubercle bacilli (espe- 

 cially bovine types). Author reported 

 that the bovine type developed after 

 about 36 hours. 



References: Valleti (1913 p. 240), Wigger 

 (1914 p. 3). 



2024. Klimmer's Casein Agar 

 Constituents: 



1. Water 1000.0 cc. 



2. Peptone 10.0 g. 



3. NaCl 0.1 g. 



4. MgS04 0.3 g. 



5. K2HPO4 0.3 g. 



6. Casein 5.0 g. 



7. Agar (2.0%) 1000.0 cc. 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Mix equal parts sterile (1) and liquid 

 sterile 2.0% agar. 



(3) Pour into plates. 

 Sterilization: Not specified. 



Use: Determine peptonizing ability. The 

 author reported that peptonizing bac- 

 teria dissolved the casein giving a light 

 area. 



Reference: Klimmer (1923 p. 203). 



2025. Mayer's Mucin Agar 



Same as medium 969, but substituting 

 nutrient agar for bouillon. 



2026. Fichtner's Sputum Agar 

 Constituents : 



1. Nutrient agar. 



2. Sputum. 



Preparation : 



(1) Obtain clean sputa and place in 

 sterile test tubes. 



(2) Immerse the tubes in a water bath so 

 that the water level in the bath is 

 higher than the sputa in the tube. 

 Do not heat over 70°C. Usually 

 60°C. is sufficient. 



(3) Heat the sputa until a homogenous 

 thin liquid is formed. Shake or stir 

 the sputa occasionally. Do not heat 

 longer than 1| to 2 hours. 



(4) Prepare slants or plates from nutrient 

 agar. 



(5) Streak the surface of agar slants or 

 plates with the sterilized sputa, in 

 the same manner as one streaks agar 

 with blood to prepare blood agar 

 slants. 



Sterilization: See preparation of steriliza- 

 tion of sputum. Method of sterilization 

 of agar not given. 



Use: Cultivation of influenza bacilli. 

 Author reported that influenza colonies 

 were generally more turbid and flatter 

 than on blood agar. 



Reference: Fichtner (1904 p. 376). 



2027. Finger, Ghon and Schlagenhaufer's 

 Urine Agar 



Constituents : 



1. Nutrient agar (2.0%). 



2. Urine. 

 Preparation : 



(1) Prepare 2.0% nutrient agar contain- 

 ing 1.0% peptone. Reaction to be 

 neutral or slightly alkaline. 



(2) Obtain urine under aseptic condi- 

 tions. The reaction is slightly acid. 



(3) Add one part sterile (2) to two 

 parts (1). The final reaction is 

 neutral. 



Sterilization: Method of sterilization of 

 agar not given. 



Use: Cultivation of gonococci. 



Variants : 



(a) The authors reported that the urine 

 may be mixed with the agar and then 

 sterilized. (Method not given.) 

 The urine may be sterilized by heat- 

 ing at 70 to 80°C. for 30 minutes and 

 then mixing one part urine with two 

 parts sterile agar. The finished 

 medium reacts neutral or slightly 

 alkaline. 



