650 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



(b) Hammer specified the use of albumin 

 urine from a nephritis patient. 

 References: Finger, Ghon and Schlagen- 

 haufer (1894 p. 14), Hammer (1895 p. 859). 



2028. Eberson's Yeast Infusion Agar 

 Constituents : 

 1- Water 100.0 cc. 



2. Nutrient agar (1.0%, with 



2.0% peptone) 100.0 cc. 



3. Potassium phosphate (0.4%) . 0.4 g. 



4. Yeast, bakers or brewers 10.0 g. 



Preparation : 



(1) Macerate 10.0 g. of bakers or brewers 

 yeast in 100.0 cc. of water for 20 

 minutes. 



(2) Steam the suspension for 2 hours at a 

 temperature not exceeding 100°C. 



(3) Clarify by adding Merck's dialized 

 iron (5.0% ferric hydroxide) and 

 filtering thru glass wool. 



(4) Add an equal amount of 1.0% agar 

 containing 2.0% peptone and 0.4% 

 potassium phosphate to (3). 



(5) Adjust the reaction to pH 7.6 (pH 7.4 

 following sterilization). 



(6) Tube in 10.0 cc. quantities. 

 Sterilization: Autoclave for 30 minutes at 



15 pounds pressure. 

 Use: Culture medium for preserving stock 



cultures of meningococci. 

 Reference: Eberson (1919 p. 852). 



SUBGROUP II-C. SECTION 7 



Basal or complete media containing agar 

 and a digest or autolysate other than a 

 commercial digest. 

 Ai. Digested by acids or bases. 

 Robinson and Rettger's Lactalbu- 



min Agar 2029 



Robinson and Rettger's Casein 

 Edestin Derivatives. Lactal- 



bumin Agar 2030 



Robinson and Rettger's Casein 



Product C Agar 2031 



Robinson and Rettger's Casein 



Product B Agar 2032 



Robinson and Rettger's Edestin 



Product Agar 2033 



Deycke and Voigtlander's Albu- 

 minate Agar 2034 



Ao. Digested by enzymes. 



Bi.* Enzymes of animal origin employed. 



Ci. Containing peptic digests only. 



* See page 651 for Bj. 



Di. Digested by means of minced stomach. 

 El. Not containing animal fluids. 

 Martin and Loiseau's Peptic Digest 



Agar 2035 



Dujarric's Orange Juice Peptic 



Digest Agar 2036 



Stickel and Meyer's Peptic Digest 



Agar 2037 



Harvey's Basal Peptic Digest Agar. 2037a 

 Besredka and Jupille's Egg Stomach 



Digest Agar 2038 



E2. Containing animal fluids. 

 Sellards and Bigelow's Veal Digest 



Blood Agar 2039 



Martin's Basal Stomach Digest Veal 



Infusion Agar 2040 



Harvey's Defibrinated Blood Digest 



Agar 2041 



Harvey's Pepsin Digest Serum Agar. 2042 

 Martin's Digest of Ascitic Fluid 



Agar 2043 



D2. Digested by means of pepsin. 

 Harvey's Pepsinized Blood Agar. . . 2044 

 Deycke and Voigtlander's Pepsinized 



Meat Agar 2045 



Kammen's Pepsinized Yeast Agar 



(Klimmer) 2046 



Jensen's Milk Digest Agar 2047 



Bacto Nutritive Caseinate Agar 



(Dehydrated) 2048 



Bacto Peptonized Milk Agar (Dehy- 

 drated) 2049 



Co.* Containing tryptic digests only. 

 Di.f Containing digests of casein or milk 



products. 

 El. Additional constituents, if any, in- 

 organic. 



Cole and Onslow's Trypsinized Ca- 

 sein Agar 2050 



Teruuchi and Hida's Trypsinized 



Casein Agar 2051 



Norris' Trypsinized Caseinogen 



Agar 2052 



Norris' Simplified Caseinogen Agar. 2053 

 Teague and Deibert's Trypsinized 



Casein Agar 2054 



E2. Containing additional organic con- 

 stituents. 

 Sierakowski's Trypsinized Casein 



Lactose Agar 2055 



Harvey's Trypsinized Caseinogen 



Blood Agar 2056 



Harvey's Blood Tryptamine Agar. . 2057 



* See C3, page 651. 

 t See page 651 for Dj. 



