CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



651 



Teague and Deibert's Serum Tryp- 



sinized Casein Agar 2058 



Kulp and Rettger's Trypsinized 



Casein Agar 2059 



Kulp and Rettger's Trypsinized 



Klim Agar 2060 



D2. Not containing digests of casein or 



milk products. 

 El. Containing digests of mutton or other 

 meats. 

 Harvey's Trypsinized Ox Heart 



Agar 2061 



Norris' Trypsinized Mutton Agar. . 2062 

 Harvey and Iyengar's Desiccated 

 Trypsinized Mutton Bouillon 



Agar 2063 



Harvey and Iyengar's Desiccated 

 Bile Salt Trypsinized Mutton 



Agar 2064 



Vardon's Desiccated Aronson's 



Agar 2065 



Deycke and Voigtlander's Tryptic 



Meat Agar 2066 



Murray and Ayrton's Heart Infu- 

 sion Agar 2067 



Hottinger's Agar (Park, Williams 



and Krumwiede) 2068 



Gordon and Hines' Trypagar 2069 



E2. Containing other than meat digests. 

 Duval and Harris' Tryptic Digest 



Agar 2070 



Distaso's Trypsinized Serum Agar. 2071 

 Stickel and Meyers' Trypsinized 



Blood Clot Agar 2072 



C3. Containing both tryptic and peptic 

 digests. 

 Stickel and Meyers' Tryptic and 



Peptic Digest Agar 2073 



Deycke and Voigtlander's Tryptic 



and Peptic Meat Digest Agar 2074 



B2. Enzymes of plant origin employed. 

 Kligler's Yeast Autolysate Agar... 2075 

 van Steenberge's Yeast E.xtract 



Agar 2076 



Jotton's Yeast Autolysate Agar 2077 



Couret and Walker's Autolyzed Tis- 

 sue Agar 2078 



2029. Robinson and Rettger's Lactalbumin 

 Agar 



Constituents : 



1. Water 1000.0 cc. 



2. Lactalbumin 20.0 g. 



3. Agar 15.0 g. 



Preparation : 



(1) Heat 100.0 g. of crude commercial 

 milk albumin containing equal 

 amounts of calcium phosphate and 

 protein for 2 to 3 hours with 200.0 cc. 

 of 10.0% HCl under reflex condenser. 



(2) Filter and heat residue with 200.0 cc. 

 10.0% HCl until no Biuret is given. 



(3) Heat in open vessel to drive off HCl. 



(4) Neutralize with NaOH. 



(5) Filter. 



(6) Evaporate filtrate to sticky paste. 

 This is lactalbumin. Add paste and 

 agar to water. Adjust to faintly 

 alkaline with litmus. Sterilize. 

 (Method not given.) 



(7) Dissolve 20.0 g. of (6) and 15.0 g. agar 

 in 1000.0 cc. of water. 



(8) Make faintly alkaline to litmus. 

 Sterilization: Method not given. 



Use: General culture media for pathogenic 

 forms. Authors reported that 2.0% lact- 

 albumin gave best results; a 4.0% medium 

 contained too much NaCl, due to neu- 

 tralization of acid by NaOH. 



Reference: Robinson and Rettger (1918 

 p. 220). 



2030. Robinson and Rettger's Casein Edes- 

 tin Derivatives, Lactalbumin Agar 



Constituents: 



1. Water 1000.0 cc. 



2. Casein product C 25.0 g. 



3. Edestin product 2.5 g. 



4. Lactalbumin product 



(paste) 2.5 g. 



5. Agar 15.0 g. 



Preparation : 



(1) Prepare casein product C as follows: 



(a) Boil over free flame under a reflux 

 condenser for 8 hours, 50.0 g. crude 

 casein in 200.0 cc. 10.0% HCl. 



(b) Distill solution by frequently add- 

 ing water. 



(c) Neutralize residue with NaOH. 



(2) Prepare edestin product by: 



(a) Heat 40.0 g. pure re-crystallized 

 edestin over a free flame with 

 10.0% HCl under a reflux con- 

 denser, until it no longer gives 

 Biuret test. 



(b) Heat for several hours in open dish 

 to drive out HCl. 



(c) Neutralize with NaOH. 



