658 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



(22) Filter while hot thru well-wetted 

 thick filter paper. 



(23) Mix one part defibrinated blood 

 with 3 parts distilled water. 



(24) Add 0.5 cc. of 10% NaOH per 100 cc. 

 (23) to (23) . (This permits steriliza- 

 tion in the autoclave without coagu- 

 lation.) 



(25) Mix one part sterile (24) with two 

 parts sterile (22) under aseptic 

 conditions. 



(26) Slant. 



Sterilization: Method of sterilization of 

 (22) not specified. Sterilize (24) at 

 112°C. in the autoclave. 



Use: Cultivation of meningococcus, etc. 



Reference: Harvey (1921-22 p. 74). 



2043. Martin's Digest Ascitic Fluid Agar 



Constituents : 



1. Martin's digest veal infusion 



agar 200.0 cc. 



2. Ascitic fluid 100.0 cc. 



Preparation : 



(1) Mix 5.0 cc. of 10.0% NaOH and 

 100.0 cc. of ascitic fluid. 



(2) Make sterile cool (1) faintly alkaline 

 to litmus by the addition of 1-15 

 HCl. 



(3) Mix one part sterile (2) at 80°C. with 

 two parts sterile Martin's Digest Veal 

 Infusion Agar (Harvey) (see medium 

 2040). 



Sterilization: Sterilize (2) and (3) at 112°C. 



in the autoclave. Method of sterilization 



of agar not given. 

 Use: General culture medium. 

 Reference: Harvey (1921-22 p. 84). 



2044. Harvey's Pepsinized Blood Agar 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Blood, ox 500.0 g. 



3. Pepsin powder 15.0 g. 



4. Agar 

 Preparation : 



(1) Mix 500.0 cc. of ox blood, 15.0 g. 

 powdered pepsin, 15.0 g. HCl and 

 1000.0 cc. distilled water. 



(2) Keep for 12 hours at 40 to 42°C. 



(3) Boil 30 minutes. 



(4) Filter. 



(5) Make faintly alkaline to litmus. 



(6) Solidify with agar. 



Sterilization: Not specified. 

 Use: General culture medium. 

 Reference: Harvey (1921-22 p. 77). 



2045. Deycke and Voigtlander's Pepsinized 

 Meat Agar 



Constituents: 



1. Distilled water 2000.0 cc. 



2. Meat, horse 125.0 g. 



3. HCl (50.0%) 2.0 cc. 



4. Pepsin (Witte's) 3.0 g. 



5. NaCl 8.0 g. 



6. Glycerol 150.0 g. 



7. Agar 

 Preparation : 



(1) Grind up 125.0 g. of lean horse meat 

 with 3.0 g. of pepsin and 400.0 g. 

 distilled water with 2.0 cc. of HCl. 



(2) Place in an Erlenmeyer flask and 

 incubate at 37°C. until there is com- 

 plete solution. 



(3) Dilute to 2 liters, neutralize with 

 NaoCOa and add 8.0 g. of NaCl. 



(4) Add 150.0 g. glycerol and sufficient 

 agar to solidify. 



Sterilization: Method not given. 



Use: Cultivation of parasitic and sapro- 

 phytic forms. 



Reference: Deycke and Voigtlander (1901 

 p. 625). 



2046. Kamen's Pepsinized Yeast Agar 

 (Klimmer) 



Constituents : 



1. Water 1000.0 cc. 



2. Yeast 



3. NaCl 3.0 g. 



4. Agar 22.0 g. 



Preparation: 



(1) Autoclave 10,000.0 g. yeast with 

 20 liters water for 2 hours at 1.5 to 

 1 atmosphere pressure. 



(2) Allow to settle and decant the 

 liquid. 



(3) Add pepsin and about 10 liters of 

 0.5% HCl solution to the thick 

 yeast residue from (2). 



(4) Incubate (3) for 5 days at 37°C. 



(5) Allow to settle and decant the 

 liquid or filter. 



(6) Neutralize the liquid by the addi- 

 tion of NaOH. 



(7) Add the liquid from (2) to (6). 



