CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



667 



may be added in the form of a strong 

 sterilized solution and added to the 

 medium after sterilization.) 

 Sterilization: Sterilize 20 minutes at 100°C. 



on each of 3 successive days. 

 Use: Desiccated medium for colon typhoid 



group. 

 Reference: Harvey and Iyengar (1921-22 

 p. 366). 



2065. Vardon's Desiccated Aronson's Agar 



Constituents : 



1. Tryptic digest of mutton. . . 1000.0 cc. 



2. Agar (9.0%) 9.0 g. 



3. Na2C03 (10.0% anhydrous 

 solution) 12.0 cc. 



4. Sucrose (20.0% solution) . . . 10.0 cc. 



5. Dextrin (20.0% solution) . . . 10.0 cc. 



6. Fuchsin, basic (sat. alcoholic 

 solution) 0.8 cc. 



Preparation : 



(1) Add 9.0% washed fiber agar to 

 1000.0 cc. of double strength tryptic 

 digest of mutton. (See variant of 

 medium 1715.) 



(2) Heat for 60 minutes at 120°C. to 

 dissolve the fiber agar. 



(3) Add 12.0 cc. 10.0% anhydrous so- 

 dium carbonate to the nutrient agar. 



(4) Steam 15 minutes at 100°C. 



(5) Add while hot 10.0 cc. 20.0%o sucrose, 

 10.0 cc. 20.0%, dextrin, and 0.8 cc. 

 saturated alcoholic solution of basic 

 fuchsin. 



(6) Allow to solidify. 



(7) Cut up the solidified medium and 

 pass thru a meat-mincing machine 

 with small outlet holes. 



(8) Spread the minced medium on metal 

 trays. 



(9) Dry at a temperature not exceeding 

 60°C. 



(10) Grind the medium when dried to a 

 powder. 



(11) Weigh the powder obtair.^d. 



(12) Complete the drying at a tem- 

 perature not exceeding 60°C. 



(13) Record the final weight. 



(14) Add 100.0 cc. water to the requisite 

 amount of (13). (See step (21) of 

 variant of medium 2063.) 



(15) Dissolve the powder by steaming 

 15 minutes at 110°C. 



(16) Add, while liquid, 2.0 cc. 10.0% 

 freshly prepared sodium sulphite. 



(17) Pour plates or prepare slopes. 

 Sterilization: See step (15) above. 

 Use: Desiccated culture medium. 

 Reference: Vardon (1923-24 p. 432). 



2066. Deycke and Voigtlander's Tryptic 

 Meat Agar 



Constituents : 



1. Water 3000.0 cc. 



2. Glycerol 190.0 g. 



3. Pancreas 



4. Meat 200.0 g. 



5. NaOH (3.0%) 300.0 cc. 



6. Agar to solidify 

 Preparation : 



(1) Allow the finelj' chopped pancreas 

 of hogs to remain on ice for 24 hours. 



(2) Mix with 40.0 g. glycerol and 

 160.0 cc. water. 



(3) Press out the fluid from this mixture. 



(4) Dissolve 200.0 g. meat in 300.0 cc. of 

 3.0% NaOH solution. 



(5) Filter (4) and neutralize. 



(6) Add 0.25% NaaCOs (siccum) to (5). 



(7) Sterilize (method not given). 



(8) Add 50.0 g. of (3) to (7). 



(9) Incubate for 7 to 10 hours at 37°C. 



(10) Neutralize with HCl. 



(11) Dilute with water to 3 liters. 



(12) Add 150.0 g. glycerol and the usual 

 amount of agar to (11). 



Sterilization: Not specified. 



Use: Cultivation of parasitic and sapro- 

 phytic forms. Authors reported that it 

 was necessary to add glucose to culti- 

 vate streptococci. Reitz used this me- 

 dium in the bacteriological examination 

 of butter for the detection of diphtheria 

 bacilli. 



References: Deycke and Voigtlander (1901 

 p. 626), Reitz (1906 p. 723). 



2067. Murray and Ayrton's Heart Infusion 

 Agar 



Constituents: 



1. Distilled water 2000.0 cc. 



2. Agar 40.0 g. 



3. Heart, ox 1000.0 g. 



4. NaCl (0.25%) 2.5 g. 



5. KCl (0.01%) 0.2 g. 



6. CaCl2 0.1 g. 



