CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



675 



Thorn's Bean Agar (Tanner) 2135 



Stutzer's Bean Infusion Agar 2136 



Vogel and Zipfel's Legume Flour 



Agar (Klimmer) 2137 



C2. Materials other than seeds used. 

 Bacto Bean Pod Agar (Dehydrated) . 2138 



Didlake's Soy Bean Root Agar 2139 



Zipfel's Legume Leaf Agar 2140 



Simon's Legume Agar (Klimmer and 



Kriiger) 2141 



2079. Gassner's Yeast Autolysate Agar 



Constituents: 



1. Water. 



2. Yeast, Brewer's. 



3. Agar (3.0%). 



4. NaCl. 

 Preparation : 



(1) Place about 10 liters of brewer's yeast 

 in a flask and wash with water. Allow 

 to stand for 30 minutes and pour off 

 the liquid. 



(2) Repeat the washing process until the 

 wash water is no longer brown, but 

 slightly turbid. Washing five times 

 is usually sufficient. 



(3) After pouring off the last water add 

 18 liters of water to the remaining 

 washed yeast cells. 



(4) Boil in the autoclave or steamer as 

 in the preparation of meat bouillon. 



(5) Allow to stand for a suitable length 

 of time and remove the liquid from 

 the sediment or filter thru filter 

 paper. 



(6) This bouillon may be used exactly 

 in the same manner as meat bouillon. 



(7) To prepare agar dissolve 30.0 g. of 

 agar and 5.0 g. NaCl in 1000.0 cc. 

 of (6). 



(8) Adjust to slightly alkaline to litmus. 

 Sterilization: Not specified. 



Use: General inexpensive culture medium. 

 Variants: The author added 0.3 or 0.5% 



lactose. 

 Reference: Gassner (1916-17 p. 311). 



2080. Harvey's Yeast Extract Agar 



Constituents : 



1. Water 1000.0 cc. 



2. Yeast (bakers or brewers) . . 100.0 g. 



3. Agar (2.5% solution) 

 Preparation: 



(1) Mix one part bakers or brewers yeast 

 with ten parts water. 



(2) Heat the mixture 20 minutes at a 

 temperature not exceeding 50°C. 



(3) Steam 2 hours. 



(4) Filter and refilter thru well-wetted, 

 thick, filter paper. 



(5) Mix two parts (4), cooled to 15°C. 

 with three parts melted 2.5% agar 

 solution of a reaction pH = 7.4. 

 (Agar may have peptone and NaCl 

 in it if desired.) 



Sterilization: Sterilize in the autoclave. 



Use: Cultivation of meningococci. Harvey 

 reported that with a semi-liquid agar 

 (0.5%) viability can be preserved in stab 

 cultures much longer than with a stiffer 

 agar. 



Reference: Harvey (1921-22 p. 120). 



2081. Beijerinck's Yeast Water Agar 



Constituents : 



1. Water tap 1000.0 cc. 



2. Yeast 80.0 g. 



3. Agar 0.75 g. 



4. Glucose 5.0 to 10.0 g. 



5. Chalk 

 Preparation: 



(1) Boil 8.0 yeast in 100.0 cc. tap water. 



(2) Dissolve 0.75 g. agar and 5.0 to 10.0 g. 

 glucose in (1) by boiling. 



(3) Filter. 



(4) Add a layer of pure chalk suspension. 

 Sterilization: Not specified. 



Use: To study the fermentation of glucose 



by bacteria. 

 Reference: Beijerinck (1891 p. 782). 



2082. Duval's Parasite Agar 



Constituents : 



1. Water 1000.0 cc. 



2. Agar 



3. Entameba coli 



4. B. typhosus 

 Preparation : 



(1) Prepare a solution of agar (amount 

 not given) in water. 



(2) Adjust to 1.0% alkaline to phenol- 

 phthalein. 



(3) Tube. 



(4) Incubate the sterile slants of (3) with 

 a mixture of Entameba coli and B. 

 typhosus. 



(5) Incubate at room temperature for 48 

 hours. 



Sterilization: Method of sterilization of 

 (3) not given. 



