676 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Use: Cultivation of B. leprae. Inoculate 

 the slant with an emulsion prepared from 

 leprous tissue. The author reported that 

 the organism grew without the presence 

 of B. typhosus. 



Reference: Duval (1910 p. 653). 



2083. Gassner's Asparagin Yeast Water 

 Agar 



Constituents : 



1. Water 900.0 cc. 



2. Yeast water 900.0 cc. 



3. Agar 30.0 g. 



4. NaCl 5.0 g. 



5. Asparagin 10.0 g. 



6. Water blue (0.5%) 250.0 cc. 



Preparation : 



(1) Prepare yeast water. 



(2) Mix equal parts yeast water and 

 water. 



(3) Dissolve 30.0 g. agar, 10.0 g. aspara- 

 gin, and 5.0 g. NaCl in (2). 



(4) To each 80.0 cc. of (3) add 20.0 cc. 

 of a 0.5% solution of water blue. 



Sterilization: Not specified. 



Use: To study the nitrogen requirements 

 for growth of the colon-typhoid and 

 dysentery group. The author reported 

 that asparagin was utilized as a nitrogen 

 source by the typhoid and dysentery 

 group as well as by Bad. coli. 



Variants : The author added 0.1, 0.5 or 1.0% 

 glucose. 



Reference: Gassner (1917-18 p. 260). 



2084. V. Eisler's Bacterial Emulsion Agar 



Constituents : 



1. Water 100.0 cc. 



2. NaCl (0.5%) 0.5 g. 



3. Agar 3.0 g. 



4. Bacterial emulsion 

 Preparation : 



(1) Dissolve 3.0% agar in a 0.5% NaCl 

 solution. 



(2) The reaction is usually slightly 

 alkaline. 



(3) Distribute in 5.0 cc. lots and sterilize 

 (method not given). 



(4) Prepare bacterial emulsions by wash- 

 ing bacteria {B. coli, typhosus, and 

 Staphylococcus pyogenes aureus were 

 used) from large 24 hour agar surfaces 

 with 25 to 30.0 cc. salt solution. 



(5) Heat the emulsion for an hour and a 

 half at 56°C. or boil for one-half hour. 



(6) Add from 1.0 to 3.0 cc. of the emulsion 

 to each sterile tube of agar melted and 

 cooled to 50°C. 



Sterilization: Method of sterilization of 

 agar not given. See step (5) for steriliza- 

 tion of bacterial emulsion. 



Use: To study growth of bacteria on killed 

 bacteria. Author reported that B. coli 

 grew only fairly well. Did not grow at 

 all on the sediment. Grew well on sta- 

 phylococcus organisms, but poorly on the 

 sediment. Scant growth of B. coli on 

 B. typhosus cells. Staphylococcus aureus 

 grew well on B. coli, typhosus or Staphy- 

 lococcus emulsion; B. typhosus grew on 

 Staphylococcus aureus emulsion, but very 

 poorly on B. coli or typhosus emulsions. 



Variants: The author specified that the 

 emulsion might be centrifuged for quite a 

 long time and the supernatant, cell free 

 opalescent fluid be added to the agar, or 

 the sediment be mi.xed with a volume of 

 NaCl solution, equal to that removed 

 from the sediment, and added to the 

 agar. 

 Reference: v. Eisler (1918 p. 197). 



2085. Lanken and Meyers' Fungus Infusion 

 Agar 



Solidify medium 1150 and variant (b) 

 medium 1150 by the addition of 2.0% agar. 



2086. Ayers and Mudge's Milk Powder 

 Yeast Agar 

 Constituents: 



1. Water 250.0 cc. 



2. Skimmed milk powder 5.0 g. 



3. Na2HP04-2H20 (Sorensen's 

 phosphate) 1 .0 g. 



4. Yeast (dry fresh) 10.0 g. 



5. Agar (3.0% washed) 500.0 cc. 



Preparation : 



(1) Pour 5.0 g. skimmed milk powder 

 into 20.0 cc. cold distilled water. 

 Stir until solution is complete. 



(2) Dissolve 1.0 g. Sorensen's phosphate 

 (Na2HP04.2H20) in 5.0 cc. distilled 

 water by heating. 



(3) Add (2) to (1), place at 30°C. in a 

 water bath and heat to eO^C. in 10 

 minutes. A flocculent grayish pre- 

 cipitate appears. 



