678 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



2090. Peklo's Malt Infusion Agar 



Same as medium 1214 but solidified by the 

 addition of 1.0% agar. 



2091, Fulmer and Grimes' Malt Infusion 

 Agar 



Constituents: 



1. Distilled water 1150.0 cc. 



2. Malt, distillers 360.0 g. 



3. Agar (1.5%) 15.0 g. 



Preparation : 



(1) Mash at 55 °C. 360.0 g. malt with 

 1150.0 CO. distilled water for 24 hours. 



(2) Filter thru towelling, then thru filter 

 paper. 



(3) Heat for 30 minutes under 15 pounds 

 pressure. 



(4) Allow to stand 3 days and filter. 



(5) Add agar. 



(6) Heat in autoclave 30 minutes at 15 

 pounds. 



(7) Filter thru absorbent cotton and 

 tube. 



Sterilization: Sterilize in live steam for 30 



minutes on 2 successive days. 

 Use: Growth of yeast Sacch. cerevisiae and 



Torula sphaerica. 

 Reference: Fulmer and Grimes (1923 p 



586). 



2092. Rettger, Reddish and McAlpine's 

 Malt Extract Agar 



Constituents: 



1- Water 900.0 cc. 



2. Malt extract powder, Difco . 100.0 g. 

 Preparation : 



(1) Dissolve 2 in 1. 



(2) Reaction is about pH = 5.5 to 5.6. 

 Sterilization: Not specified. 



Use: Isolation of yeast from feces. 

 Authors reported that the reaction was 

 too acid for the development of bacteria. 



Reference: Rettger, Reddish and McAlpine 

 (1924 p. 328). 



2093. Tanner and Deck's Acid Near Beer 



Agar 

 Constituents : 



1. Distilled water 600.0 cc. 



2. Malt drink (near beer) 400.0 cc. 



3. Agar 15.0 g. 



4. Lactic acid (5.0% soln.) 



Preparation : 



(1) Add 15.0 g. agar to 400.0 cc. malt 

 drink (near beer) and 600.0 cc. dis- 

 tilled water. 



(2) Boil to dissolve the agar, 



(3) Tube. 



(4) Just previous to use add 0.4 cc. of 

 5.0% sterile solution of lactic acid to 

 each sterile tube of (3). 



Sterilization: Method of sterilization of 

 agar or lactic acid not given. 



Use: Cultivation of yeast causing sore 

 throat probably Endomyces or Monilia. 



Reference: Tanner and Deck (1923-24 p 

 285). 



2094. Janke's Lager Beer Agar 

 Constituents : 



1. Lager beer. 



2. Gelatin. 



3. Alcohol. 



4. Agar. 

 Preparation: 



(1) Evaporate lager beer to one-half its 

 original volume by steaming. 



(2) Add water to its original volume. 



(3) Add 2.0% agar and 2.0% gelatin to 

 (2). 



(4) Clarify with white of egg. 



(5) Filter thru cotton. 



(6) Make up to the original volinne by 

 the addition of water. 



(7) Distribute in 10.0 cc. lots in Freuden- 

 reich flasks. 



Sterilization: Sterilize on each of 3 succes- 

 sive days in the steamer. 



Use: Cultivation of acetic acid bacteria 

 from beers. 



Reference: Janke (1916 p. 6). 



2095. Sobel's Lactose Beer Agar 



Constituents: 



1. Water 500.0 cc. 



2. Beer 500.O cc. 



3. Agar 15.0 g. 



4. Lactose 5.0 g. 



5. Congo red 1.0 g. 



Preparation : 



(1) Boil 500.0 cc. of beer, (preferably 

 stale Pilsner beer) for 5 minutes. 



(2) Cool and bring to 1 liter volume by 

 the addition of water. 



(3) Add 15.0 g. agar and allow to soak for 



