CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



679 



2 hours. Then boil until the agar has 

 dissolved. 



(4) Add 5.0 g. of dry sterilized lactose to 

 boiling (3) and 1.0 g. of dry sterilized 

 Congo red. 



(5) Boil vigorously for several minutes 

 and add the amount of water lost by 

 evaporation. 



(6) Pour directly into sterile Petri dishes 

 if the medium is to be used at once. 



Sterilization: If the medium is not to be 



used at once sterilize in the steamer on 



two successive days. 

 Use: Diagnosis of cholera, typhoid fever 



and dysentery. The author gave the 



following reactions: 



Variants: The author used 1.0 g. of litmus 



puriss instead of congo red. 

 Reference: Sobel (1915 p. 1573). 



2096. Difco Wort Agar (Dehydrated) 



Constituents : 



1. Distilled water 



2. Maltose, Technical, Difco.... 12.75 g 



3. Malt extract, Difco 15.0 g 



4. Dextrin, Difco 2.75 g 



5. Glycerol, C. P 2.35 g 



6. K2HPO4 10 g 



7. NH4CI 10 g 



8. Peptone, Bacto 0.78 g 



9. Agar, Bacto 15-0 g 



Preparation : 



(1) Dissolve 50.63 g. Difco Wort Agar 

 (Dehydrated) in 1000.0 cc. distilled 

 water by boiling or autoclaving. 



(2) Distribute as desired. 



(3) If sterilized at 15 pounds pressure for 

 20 minutes pH = 4.7±. 



Sterilization: Sterilize at 15 pounds for 20 



minutes. 

 Use: Cultivation of yeasts. 

 Reference: Digestive Ferments Co. (1925 



p. 14). 



2097. Meacham, Hopfield and Acree's Corn 

 Meal Agar 



Constituents : 



1. Water 1000.0 cc. 



2. Corn meal, yellow 7.5 g. 



3. Agar • 25.0 g. 



4. KjHPOi to M/50 



5. Acetic acid to M/50 

 Preparation: 



(1) Cook 7.5 g. yellow corn meal with 

 200.0 cc. water in water bath at 60° C. 

 for one hour. 



(2) Allow meal to settle, decant the 

 liquid and mix fresh water with the 

 meal. 



(3) Decant this liquid and mix the two 

 extracts. 



(4) Dissolve 3, 4 and 5 in (3) and make up 

 to 1000.0 cc. 



(5) Reaction of about pH = 5.7. 

 Sterilization: Not specified. 



Use: Study phosphate-acetate and phos- 

 phate-phthalate as buffers. Authors re- 

 ported that potassium acid phthalate 

 showed best growth of E. parasitica. 



Variants: The authors used M/50 potas- 

 sium acid phthalate instead of M/50 

 acetic acid. 



Reference: Meacham, Hopfield and Acree 

 (1920 p. 307). 



2098. Buchanan's Silage Agar 



Constituents : 



1. Water, tap 1000.0 cc. 



2. Silage 500.0 g. 



3. Agar (1.5%) 15.0 g. 



Preparation : 



(1) Boil 500.0 g. fresh silage for 30 minutes 

 with a liter of tap water. 



(2) Filter. Wash the silage on the filter 

 with hot water until a liter of the 

 decoction is secured. 



(3) Autoclave with 1.5% agar until 

 solution is complete. 



(4) Filter. 



(5) Tube. 



Sterilization: Method not given. 



Use: Cultivation of Monascus purpurens 



from silage. 

 Reference: Buchanan (1910 p. 100). 



2099. Reed and Cooley's Corn Meal Agar 



Constituents: 



1. Water 500.0 cc. 



