680 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



2. Corn meal 50.0 g. 



3. Agar (1.5 or 2.0%) .... 7.5 to 10.0 g. 

 Preparation : 



(1) Allow 50.0 g. of corn meal to simmer 

 in 500.0 cc. of water for two hours. 

 {Phaseolus lunatus, lime bean meal 

 may be used instead of corn meal.) 



(2) Strain thru a cloth. 



(3) Add 1.5 to 2.0% agar. 



(4) Melt and filter thru cotton. 

 Sterilization: Sterilize fractionally on three 



successive days. 

 Use: Cultivation of Heterosporiutn vari- 



ahile. Authors reported this a very good 



medium for fungi. 

 Reference: Reed and Cooley (1911-12 p. 



49). 



2100. Barlow's Sucrose Corn Agar (Tanner) 



Constituents: 



1. Water 4000.0 cc. 



2. Corn 1000.0 g. 



3. Sucrose 80.0 g. 



4. Agar 60.0 g. 



5. Litmus 100.0 g. 



Preparation : 



(1) Stir 1000.0 g. of corn into boiling 

 water. 



(2) Add 3, 4 and 5 to (1). 



(3) Boil 5 minutes. 



(4) Heat in an autoclave and cool slowly. 

 The heavy matter settles off. 



(5) Draw ofT and tube the supernatant 

 agar. 



Sterilization: Not specified. 

 Use: General culture medium. 

 Reference: Tanner (1919 p. 50). 



2101. Pinoy's Flax Seed Agar 



Constituents : 



1. Water 1000.0 cc. 



2. Agar 20.0 g. 



3. Flax seed 50.0 g. 



Preparation: 



(1) Prepare a medium from 1 liter of 

 water, 20.0 g. of agar and 50.0 g. 

 flaxseed. (Method not given.) 



(2) Heat at 117°C. 



(3) Distribute in^culture apparatus. 

 Sterilization: Sterilize at 115°C. for 15 



minutes. 

 Use : Cultivation of Bacterium fimhriatum. 

 Reference: Pinoy (1907 p. 626). 



2102. Omeliansky and Ssewerowa's Flax 



Fiber Agar 

 Constituents : 



1- Water iqqo.O cc. 



2. Flax fiber 50.0 g. 



3. Mannitol 20. g. 



4. Potassium phosphate 0.2 g. 



5- Agar 15 og. 



Preparation : 



(1) Heat 50.0 g. of flax at 115°C. in the 

 autoclave with a liter of water 



(2) Filter. 



(3) Neutralize to slightly alkaline re- 

 action by adding NaoCOa. 



(4) Dissolve 3, 4 and 5 in (3). 

 Sterilization: Not specified. 



Use: To study pigment production of 

 azotobacter, Azotobacter chroococcum. 

 Authors reported that on the medium 

 given above brown pigment appeared 

 after ten days. The substitution of flax 

 extract for water increased the luxuriance 

 of growth of Azotobacter, Clostridium, 

 Pasteurianum, Bac. cellulose, Nitrobac- 

 ter and other forms. 



Variants: The authors reported that using 

 a medium prepared as above but sub- 

 stituting 20.0 to 30.0 g. of dextrin for 

 mannitol, and adding 20.0 g. of chalk that 

 pigment was produced after 3 to 4 days. 

 The addition of the chalk favored the 

 production of pigment. Pigment more 

 luxuriant in the variant than in the 

 medium given above. 

 Reference: Omeliansky and Ssewerowa 

 (1911 p. 645). 



2103. Heider's Wheat Agar (Klimmer) 

 Constituents: 



1. Water 1000.0 cc. 



2. Wheat 500.0 g. 



3. Agar (1.5%) 15.0 g. 



Preparation : 



(1) Soak 500.0 g. of wheat in a liter of 

 water for 24 hours. 



(2) Filter. 



(3) Dissolve 1.5% agar in the filtrate. 



(4) Neutralize to litmus. 

 Sterilization: Not specified. 



Use: Spore production by Anthrax bacilli. 

 Variants: Klimmer prepared a similar 



medium as follows : 



(1) Macerate 500.0 g. of wheat with a 

 liter of water for 24 hours. 



