CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



681 



(2) Filter. 



(3) Make up the filtrate to a liter. 



(4) Dissolve 5.0 g. NaCl and 17.5 g. of 

 agar in (3). 



(5) Make slightly alkaline to phenol- 

 phthalein. 



(6) Sterilization not specified. 

 Reference: Klimmer (1923 p. 207). 



2104. Klimmer's Oat Agar 



Same as the variant of medium 2103, but 

 using oats instead of wheat. 



2105. Bacto Corn Meal Agar (Dehydrated) 



Constituents: 



1. Distilled water 



2. Corn Meal Agar (Bacto).... 20.0 g. 

 Preparation : 



(1) Dissolve 20.0 g. Bacto corn meal agar 

 (Dehydrated) in 1000.0 cc. distilled 

 water by boiling or autoclaving, 

 preferably the latter. Bacto corn 

 meal agar is a synthetic medium 

 representing the water soluble in- 

 gredients of corn meal solidified by 

 the addition of 1.5% agar. 



(2) Distribute as desired. 

 Sterilization: Sterilize for 20 minutes at 15 



pounds pressure. 

 Use: General culture medium. 

 Reference: Digestive Ferments Co. (1925 



p. 17). 



2106. Milburn's Basal Prune Agar 



Constituents: 



1. Water 1000.0 cc. 



2. Agar 



3. Prunes 1 lb. 



Preparation : 



(1) Soak 1.0 pound of dry prunes in 1 liter 

 of water for 12 hours. 



(2) Filter thru a towel and then cotton. 



(3) Concentrate to a specific weight of 

 1.06 to 1.08. 



(4) Prepare media using 40.0% of the 

 prune infusion, one of the added 

 nutrients and solidify with agar 

 (method not given) . 



Sterilization: Not specified. 



Use: To study color formation. 



Added nutrients: The author added one 



of the following materials and gave the 



following reactions : 



(a) glucose 1.0, 2.0 or 5.0%. 



(b) tartaric acid 0.2 or 0.5%. 



The glucose seemed to suppress the 

 conidia development. There was no 

 yellow color with either glucose or 

 tartaric acid. Color was dark green 

 using tartaric acid. 



(c) NH4XO3 0.5or 1.0%. 



(d) KNOs 0.5 or 1.0%. 



(e) K3PO4 0.5 or 1.0%. 



(f) K2HPO4 0.5 or 1.0%. 



(g) KH2PO4 0.5 or 1.0%. 



(h) Dipotassium ammonium phosphate. 



(i) tyrosine. 



(j) leucine. 



(k) oxamide. 



(1) legrimin. 



(m) casein. 



(n) peptone. 



(p) blood serum. 



(q) meat extract. 



(r) asparagin. 



(s) acetamide. 



(t) creatin. 



(u) albumin. 



(v) glucose. 



(w) nuclean. 



Good growth and conidia were 

 colored dirty green and greyish white 

 with tyrosine, asparagin, leucine and 

 acetamide. Little growth and no 

 color with oxamide. Creatin, legu- 

 min albumin, casein and blood serum 

 gave good growth a greenish and 

 yellow color. Remaining materials 

 gave excellent growth of a yellow 

 color. 



(x) glucose 5.0 to 40.0%. 



High concentrations of glucose 

 tended to slow up growth and no 

 color was formed. 



(y) Knops' nutrient solution 0.25, 0.5, 



1.0, 2.0 or 5.0%. 

 (z) Knops' nutrient solution 0.25, 0.5, 

 1.0, 2.0 or 5.0% + glucose (amount 

 of glucose not given). 

 The presence of Knops' mineral salts 

 seemed to aid the conidia production. 

 Hence more greenish color was pro- 

 duced. More green was produced 

 without dextrose than with it present. 

 Reference: Milburn (1904 p. 133). 



2107. Reed and Cooley's Prune Agar 



Constituents : 



1. Water 600.0 cc. 



