684 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



carried out under the water as far as 

 possible. 



(3) Allow to stand for 3 hours at room 

 temperature. 



(4) Filter thru a clean towel. 



(5) Heat at 100°C. for one hour. 



(6) Filter thru filter paper. 



(7) Dissolve NaCl and agar (amounts not 

 given) in (6). 



(8) Adjust to the desired reaction by the 

 addition of soda. 



Sterilization: Sterilize for one hour in the 

 autoclave (temperature or pressure not 

 given). 

 Use: Inexpensive culture medium. 

 Variants: The potato water may be steri- 

 lized for an hour in the autoclave and 

 kept until ready for use, and then add 

 the NaCl and agar as above. 

 Reference: Gaehtgens (1916 p. 46). 



2116. Bacto Potato Dextrose Agar 

 (Dehydrated) 

 Constituents: 



1. Distilled water 



2. Potato infusion, dry 5.0 g. 



3. Glucose, Bacto 20.0 g. 



4. Agar, Bacto 15.0 g. 



Preparation : 



(1) Dissolve 40.0 g. of Bacto Potato 

 Dextrose Agar (Dehydrated) in 

 1000.0 cc. of distilled water by boiling 

 or autoclaving, preferably the latter. 

 The dry potato infusion is prepared 

 by infusing 50. parts dried potatoes. 



(2) Distribute. 



Sterilization: Sterilize at 15 pounds pres- 

 sure for 20 minutes. 



Reference: Digestive Ferments Co. (1925 

 p. 17). 



2117. Kellerman and McBeth's Potato Agar 

 Constituents: 

 1- Water 1000.0 cc. 



2. Potato 100.0 g 



3. K2HPO4 l.Og. 



4. MgS04 l.Og. 



5. NaCl l.Og 



6. (NH4)2S04 2.0 g. 



7. CaC03 2.0 g. 



S. Agar 10.0 g. 



Preparation : 



(1) Peel, steam and mash a quantity of 

 potatoes. 



(2) To 100.0 g. of (1) add 800.0 cc. of water 

 and steam for 30 minutes. 



(3) Filter thru cotton. 



(4) Dissolve 3, 4, 5, 6 and 7 in 1000.0 cc. of 

 water. 



(5) Mix 500.0 cc. of (4) and 500.0 cc. of the 

 filtrate from (3). 



(6) Dissolve 10.0 g. of agar in (5). 

 Sterilization: Not specified. 



Use: Cultivation of Bacillus flavigens, 

 Bacillus amylolyticus, Bacillus rossica. 



Variants: Tanner used 15.0 g. agar instead 

 of 10.0 g. 



References: Kellerman and McBeth (1912 

 p. 487), Tanner (1919 p. 60). 



2118. Lubinski's Glycerol Potato Agar 

 Constituents : 



1- Water 1500.0 cc. 



2. Potato 1000.0 g. 



3. Glycerol 60.0 g. 



4. Agar 15.0 to 20.0 g. 



Preparation : 



(1) Wash potatoes clean and cut into 

 small pieces. 



(2) Boil for 3 or 4 hours under a free 

 flame or in a steamer. 



(3) Filter. 



(4) Add 1.0 to 1.5% agar. 



(5) Boil to dissolve agar and filter. 



(6) Add 4.0% glycerol. 



(7) Neutralize or may be used without 

 neutralization. 



Sterilization: Not specified. 



Use: Cultivation of tubercle bacilli. 



Reference: Lubinski (1895 p. 126). 



2119. Picker's Glycerol Potato Juice Agar 

 Constituents : 



1. Potato juice 1000.0 cc 



2. Agar (1.0%) lO.O g. 



3. Glycerol (2.0%) 20.0 cc. 



Preparation : 



(1) Method of preparation of potato 

 juice not given. 



(2) Dissolve 1.0% agar in (1). 



(3) Add 2.0% glycerol to (2). 



(4) Reaction may be adjusted neutral, 

 or slightly acid or alkaline. 



Sterilization: Not specified. 



Use: Cultivation of tubercle bacilli. The 

 author reported that best growth was 

 obtained on a neutral or slightly alkaline 

 medium. 



