CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



685 



Variants: Klimmer gave the following 

 method of preparation: 



(1) Thoroly wash and scrub medium 

 sized, sound unimpaired potatoes 

 with a scrubbing brush, soap and 

 water. 



(2) Remove the peeling and wash in 

 running tap water. 



(3) Grind the potatoes to a pulp and press 

 out the juice. 



(4) Dissolve 40.0 parts glycerol and 15 

 parts agar in 1000 parts (3) by 

 heating. 



(5) Do not filter, clarify or neutralize. 



(6) Tube. 



(7) Sterilize (method not given). 

 References: Ficker (1900 p. 509), Klimmer 



(1923 p. 206). 



2120. Weinzirl's Sucrose Potato Water Agar 



Constituents : 



1. Water 800.0 cc. 



2. Potato water 200.0 cc. 



3. Sucrose 10.0 g. 



4. Agar 20.0 g. 



Preparation : 



(1) Mix 200.0 cc. of potato water and 800.0 

 cc. water. 



(2) Dissolve 3 and 4 in (1). 

 Sterilization: Not specified. 

 Use: Cultivation of molds. 

 Reference: Weinzirl (1917 p. 441). 



2121. Rochaix's Carrot Agar 



Constituents : 



1. Water 800.0 cc. 



2. Carrot juice 200.0 cc. 



3. Agar 30.0 to 35.0 g. 



Preparation : 



(1) Wash carrots well, chop and press out 

 the juice. 



(2) Dilute 200.0 cc. of the carrot juice 

 with 800.0 cc. water. 



(3) Add 30.0 to 35.0 g. of agar to (2). 



(4) Heat in the autoclave for 20 to 25 

 minutes. 



(5) Clarify by the addition of egg white. 



(6) Make alkaline (indicator not 

 specified). 



(7) Filter thru paper. 



(8) Distribute into tubes. 

 Sterilization: Sterilize at 108 or 110°. 



(Time not given.) 



Use : General culture medium. The author 

 reported that the addition of glycerol 

 favored the growth of some organisms 

 (tubercle bacilli). 



Variants : 



(a) The author added 10.0% glycerol to 

 the agar. 



(b) Harvey prepared a similar medium as 

 follows: 



(1) Mince finely well washed carrots. 



(2) Press out the juice from the minced 

 carrots. 



(3) Mix one part water with four parts 

 carrot juice. 



(4) Add agar to 3.0%. 



(5) Steam for 45 minutes. 



(6) Clear with white of egg. 



(7) Adjust the reaction. 



(8) Filter. 



(9) Distribute into test tubes. 

 (10) Sterilize. 



References: Rochaix (1913 p. 604), Harvey 

 (1921-22 p. 119). 



2122. Schardinger's Hay Infusion Agar 



Constituents: 



1. Water 1000.0 cc. 



2. Hay 30.0 to 40.0 g. 



3. Agar (1.0 to 1.5%) . . . 10.0 to 15.0 g. 

 Preparation : 



(1) Boil 30.0 to 40.0 g. of hay with one 

 liter of water. 



(2) Filter. 



(3) Add 1.0 to 1.5% agar to (2). 



(4) Boil until agar is dissolved. 



(5) Add NajCOa until the reaction ia 

 alkaline to litmus. 



Sterilization: Method not given. 



Use: Cultivation of protozoa. Wolbach 

 and Binger cultivated Spirocheta elusa 

 (free living) on a similarly prepared 

 medium. 



Variants : Wolbach and Binger mixed equal 

 parts of an unheated filtered hay infusion, 

 see medium 1179, and a melted and cooled 

 3.0% agar jelly. 



References: Schardinger (1896 p. 541), Wol- 

 bach and Binger (1914 p. 10). 



2123. Bordet and Gengou's Potato Blood 

 Agar (Roos) 



Constituents : 



1. Distilled water 200.0 cc. 



2. Glycerol (4.0%) 8.0 g. 



