CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



687 



(5) Autoclave to dissolve. 



(6) Filter. 



(7) Tube. 



(8) Sterilize in the autoclave for 30 

 minutes at 15 pounds pressure. 



(9) When ready for use add an equal 

 quantity of blood, or more often, 

 one part blood to four parts agar. 



References: Roos (1916 p. 68), Bordet and 

 Gengou (1920 p. 734), Bezan^on (1920 p. 

 121), Harvey (1921-22 p. 73), Klimmer 

 (1923 p. 206), Povitzky (1923 p. 8), Park, 

 Williams and Krumwiede (1924 p. 122). 



2124. Schardinger's Straw Infusion Agar 



Constituents: 



1. Water 1000.0 cc. 



2. Straw 30.0 to 40.0 g. 



3. Agar (1.0 to 1.5%)... 10.0 to 15.0 g. 

 Preparation: 



(1) Same as medium 2122 but substituting 

 straw for hay. 

 Sterilization: Method not given. 

 Use: Cultivation of protozoa and amoeba. 

 Variants: 



(a) Tsujitani prepared a similar medium 

 as follows: 



(1) Cut straw real fine. 



(2) Boil a mixture of 30.0 g. (1), 10.0 g. 

 Gigartina prolifera (Tsunomato) 

 and 1000.0 cc. water in a Koch 

 steamer for one hour. 



(3) Filter thru a fine cloth. 



(4) Add 1.0% to 1.5%, agar and 10.0 cc. 

 normal soda solution. 



(5) Boil for 30 to 40 minutes. 



(6) Distribute into test tubes and 

 sterilize (method not given). 



(b) Harvey solidified 1000.0 cc. of a straw 

 decoction with 15.0 g. agar. (See 

 medium 1178 for the preparation of a 

 straw decoction.) 



References: Schardinger (1896 p. 541), 

 Tsujitani (1898 p. 667), Harvey (1921- 

 22 p. 121). 



2125. Reed and Cooley's Spinach Agar 



Constituents: 



1. Water 500.0 cc. 



2. Spinach 100.0 g. 



3. Agar (2.0%) 10.0 g. 



Preparation : 



(1) Steep 100.0 g. of young spinach leaves 

 in 500.0 cc. of water. 



(2) After it is well steeped filter and add 

 2.0% agar. 



(3) Dissolve the agar and filter thru 

 cotton. 



Sterilization: Sterilize by the intermittent 

 method using the steamer. 



Use: Cultivation of Heterosporium vari- 

 abile. The authors reported that this 

 was a good medium for the production of 

 spores for the hanging drop cultures, 

 since the fungus grew very short aerial 

 hyphae. 



Reference: Reed and Cooley (1911-12 p. 

 50). 



2126. Owen's Cane Juice Agar 



Constituents: 



1. Cane juice (raw) 1000.0 cc. 



2. Agar (2.0%) 20.0 g. 



Preparation : 



(1) Heat fresh raw cane juice. 



(2) Filter thru cotton. 



(3) Solidify with 2.0%, agar. 

 Sterilization: Not specified. 



Use: Bacterial count in cane sugar 



products. 

 Reference: Owen (1914 p. 337). 



2127. Owen's Molasses Agar 



Constituents: 



1. Water 1000.0 cc. 



2. Molasses (Final 75 Brix) . . . 160.0 g. 



3. Agar 20.0 g. 



Preparation : 



(1) Dissolve 160.0 g. final molasses 75 

 Brix and 20.0 g. agar in 1000.0 cc. of 

 water. 

 Sterilization: Not specified. 

 Use: Bacterial count in cane sugar 



products. 

 Reference: Owen (1914 p. 338). 



2128. Le Fevre and Round's Cucumber 

 Juice Agar 



Constituents : 



1. Cucumber juice 1000.0 cc. 



2. Agar (amount not given) 



3. NaCl 100.0 g. 



Preparation : 



(1) Method of obtaining cucumber juice 

 not given. 



(2) Dissolve 2 and 3 in (1). 

 Sterilization: Not specified. 



