688 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Use: Cultivation and isolation of halo- 

 philic bacteria. Authors reported that 

 organisms grew just as well on meat 

 extract with 5.0% salt. 



Reference: Le Fevre and Round (1919 p 

 178). 



2129. Bacto Lima Bean Agar (Dehydrated) 



Constituents : 



1. Distilled water 



2. Lime bean infusion (dry)... 8.0 g. 



3. Agar, Bacto 15.0 g. 



Preparation : 



(1) Dissolve 23.0 g. of Bacto Lima Bean 

 Agar (Dehydrated) in 1000.0 cc. of 

 water, by boiling or autoclaving, 

 preferably the latter. The dry lima 

 bean infusion is prepared from an 

 infusion of 50 parts dried lima beans. 



(2) Distribute as desired. 

 Sterilization: Sterilize by autoclaving for 



20 minutes at 15 pounds pressure. 

 Use: General culture medium. 

 Reference: Digestive Ferments Co. (1925 



p. 17). 



2130. Stutzer's Basal Legume Seed 

 (Infusion) Agar 

 Constituents : 



1. Distilled water 1000.0 cc. 



2. Legume seed 10.0 g 



3- Agar 10.0 g. 



Preparation : 



(1) Same as for medium 1166, but solidified 



by the addition of 1.0% agar. 

 Sterilization: Not specified. 

 Use: Cultivation of nodule bacteria. 

 Variants : Zipfel prepared a similar medium 



as follows : 



(1) Mix 100.0 g. of powdered legume seed 

 with 100.0 cc. N/1 KOH in a mortar. 



(2) Add 5 liters of water and allow to 

 stand for 24 hours. 



(3) Pour off the clear supernatant fluid. 



(4) Neutralize with H3PO4 and make up 

 to 5 liters volume. 



(5) To 1 liter of (4) add 30.0 g. of agar and 

 20.0 g. dextrose. 



(6) Acidify by the addition of 10.0 cc. 

 N/1 malic acid. 



(7) Sterilization not specified. 

 References: Stutzer (1900 p. 898), Zipfel 



(1911-12 p. 10). 



2131. Wyant and Tweed's Pea Agar 

 Constituents: 



1- Water 4000.O cc. 



2. Peas (canned) 1000.0 g. 



3- Agar qqq g 



4. Litmus 

 Preparation : 



(1) Boil 3 in 1 until the agar is dissolved. 



(2) Make up to water lost by boiling. 



(3) Mash 1000.0 g. canned peas in their 

 juice and add to (2). 



(4) Autoclave for 30 minutes at 15 pounds 

 pressure. 



(5) Allow the agar to cool slowly. This 

 allows the hulls to sink to the bottom. 



(6) Cut away and discard the sediment 

 after the agar has solidified. 



(7) Melt the remaining clear agar and 



color with litmus. 

 Sterilization: Sterilize in the autoclave. 

 Use: Cultivation of organisms causing flat 



sour in peas. 

 Reference: Wyant and Tweed (1923 p. 12). 



2132. Kaufmann's Jequirity Seed Agar 

 Constituents : 



1- Water lOO.O cc. 



2. Jequirity seeds 10.0 g. 



3. Agar 1.5 to 2.0 g. 



Preparation : 



(1) Grind 10.0 g. Jequirity seeds in a 

 mortar. 



(2) The peeled or shelled seeds now weigh 

 about 8.0 g. 



(3) Add to 100.0 cc. of water. 



(4) Boil in a steam sterilizer for about 2 

 hours. 



(5) Cool and filter. 



(6) Dissolve agar in (5). 



(7) Reaction is neutral or slightly 

 alkaline. 



(8) Distribute into test tubes. 

 Sterilization: Sterilize in the usual manner 



(Method not given). 

 Use: Cultivation of Bacillus pyocyaneus 



and many others. Author reported that 



the medium supported the growth of a 



great variety of organisms. 

 Reference: Kaufmann (1891 p. 66). 



2133. Maze's Sucrose Bean Agar 

 Constituents : 

 1. Water 1000.0 cc. 



