CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



689 



2. Beans (white) 



3. Sucrose (2.0%) 20.0 g. 



4. NaCl (1.0%) 10.0 g. 



5. Agar (1.5%) 15.0 g. 



Preparation : 



(1) Same as medium 1162 but solidified by 

 the addition of 1.5% agar. 

 Sterilization: Not specified. 

 Use: To study nitrogen fixation by bacteria 



from nodules of leguminous plants. 

 Variants : de Rossi Gino prepared a similar 



medium as follows: 



(1) Prepare agar using bean extract 

 (method not given). 



(2) Add 2.0% saccharose and 0.25% 

 NaCl. 



(3) Distribute in long necked flasks. 



(4) Adjustment of reaction or steriliza- 

 tion not specified. 



References: Maz6 (1897 p. 45), de Rossi 

 Gino (1909) (1910 p. 271). 



2134. Reed and Cooley's Lima Bean Agar 



Same as medium 2099, but Phaseolus 

 lunatus, lima bean meal was used instead 

 of corn meal. 



2135. Thorn's Bean Agar (Tanner) 



Constituents : 



1. Water 1000.0 cc. 



2. Bean, white 200.0 g. 



3. Agar as desired 



Preparation: 



(1) Heat common white beans with 5 

 volumes of water. Boiling is stopped 

 just before the swelling of the coty- 

 ledons rupture the seed coats. 



(2) Filter. This solution filters easily. 



(3) Add agar as desired. 

 Sterilization: Not specified. 



Use: General culture medium. Tanner 

 reported that this medium contained 

 sufficient nutrients to grow many species 

 normally. This medium is poor in 

 available carbon. It may be desirable to 

 add carbohydrates for certain species. 



Reference: Tanner (1919 p. 50). 



2136. Stutzer's Bean Infusion Agar 



Same as variant (c), medium 1166, but 

 solidified with 1.0% agar. 



2137. Vogel and Zipfel's Legume Flour Agar 



(Klimmer) 



Constituents: 



1. Water 1000.0 cc. 



2. Bean flour 50.0 g. 



3. Agar 30.0 g. 



4. Glucose 20.0 g. 



Preparation : 



(1) Add water to 50.0 g. of bean flour to 

 make a thick paste. 



(2) Dilute (1) to a liter by the addition 

 of water. 



(3) Allow to stand for 24 hours, shaking 

 often. 



(4) Remove the liquid from the sediment. 



(5) Filter. 



(6) Make up to one liter. 



(7) Dissolve 30.0 g. agar and 20.0 g. glu- 

 cose in (6). 



(S) Do not adjust the reaction. 

 Sterilization: Not specified. 

 Use: Cultivation of nodule bacteria. 

 Variants : Klimmer used pea flour instead of 



bean flour. 

 Reference: Klimmer (1923 p. 229). 



2138. Bacto Bean Pod Agar (Dehydrated) 



Constituents : 



1. Distilled water 



2. Agar Bacto 15.0 g. 



3. Bean Pod Meal Infusion 



(dry) 7.5 g. 



Preparation : 



(1) Dissolve 22.5 g. of Bacto Bean Pod 

 Agar (Dehydrated) in 1000.0 cc. of 

 distilled water by boiling or auto- 

 claving, preferably the latter. Bean 

 Pod Meal is prepared by infusion of 

 20 parts bean pod meal. 



(2) Distribute as desired. 

 Sterilization: Heat in the autoclave for 20 



minutes at 15 pounds pressure. 

 Use: General culture medium. 

 Reference: Digestive Ferments Co. (1925 



p. 17). 



2139. Didlake's Soy Bean Root Agar 



Constituents : 



1. Water 1000.0 cc. 



2. Soy bean root 



3. Sucrose (5.0%) 5.0 g. 



