690 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



4. Asparagin (0.25%) 2.5 g. 



5. Agar 

 Preparation : 



(1) Prepare a soy bean root infusion agar. 



(2) Dissolve 0.5% sucrose and 0.25% 

 asparagin in (1). 



Sterilization: Not specified. 



Use: Cultivation of a red pigment produc- 

 ing bacillus isolated from water. Author 

 reported that the bacillus produced a 

 red pigment only on this medium. Will 

 grow more or less luxuriantly on soy bean 

 agar leaving out the asparagin or sucrose, 

 on ordinary beef peptone agar with 0.25% 

 asparagin or 0.5% sucrose added or both 

 asparagin and sucrose, or on a plain 

 peptone water agar. On these media 

 there was no pigment production. 



Reference: Didlake (1905 p. 194). 



2140. Zipfel's Legume Leaf Agar 



Constituents : 



1. Water 1000.0 cc. 



2. Clover plant 1000.0 g. 



3. Agar 30.0 g. 



4. Glucose 20.0 g. 



Preparation : 



(1) Crush 1000.0 g. of closely picked 

 clover leaves, (or other legume) to a 

 pulp. 



(2) Extract with hot water until finally 

 10 liters of liquid results. 



(3) Filter the decoction when cold. 



(4) Concentrate the filtrate to about 

 900.0 cc. 



(5) Neutralize with N/1 NaOH. 



(6) Make up to 1.0 liter. 



(7) Distribute into small flasks. This is 

 a concentrated stock solution. 



(8) Dissolve 30.0 g. agar in 800.0 cc. 

 water by heating. 



(9) Add 20.0 g. glucose and 50.0 cc. of 

 sterile (7) to (8). 



(10) Adjust the reaction to neutral. 



(11) Make up to 1 liter volume. 

 Sterilization: Sterilize (7) in the steamer. 



Sterilization of final medium not given. 



Use: Cultivation of nodule bacteria. 



Variants : The medium may be utilized with 

 a neutral reaction, or add 10.0 cc. of N/1 

 malic or citric acid or 10.0 cc. of N/1 

 NaOH to give an alkaline medium. 



Reference: Zipfel (1911-12 p. 106). 



2141. Simon's Legume Agar (Klimmer and 

 Kriiger) 



Constituents: 



1. Water 1000.0 cc. 



2. Agar 15.0 g. 



3. Legimae infusion 2.0 cc. 



4. Asparagin 10.0 g. 



5. K2CO3 

 Preparation : 



(1) Prepare the legume infusion by pour- 

 ing hot water over finely chopped 

 straw of the legumes, mix well, 

 macerate for two days and then boil 

 an hour. Pour ofl the liquid, filter 

 and evaporate until it contains 10.0% 

 dry material. 



(2) Dissolve 2 and 4 in 1000.0 cc. of water. 



(3) Add a knife point of K2CO3 and 2.0 

 cc. of (1) to (2). 



(4) Neutralize by the addition of NaOH 

 using litmus as an indicator. 



(5) Add malic acid to give a distinct acid 

 reaction. 



Sterilization: Not specified. 



Use: Cultivation of nodule bacteria from 



leguminous plants. 

 Reference: Klimmer and Kriiger (1914 p. 



258), Klimmer (1923 p. 228). 



SUBGROUP II-C. SECTION 9 



Basal or complete media containing agar, 

 with constituents of animal origin (exclu- 

 sive of digests) of unknown chemical 

 composition. 

 Ai. Containing animal products exclusive 



of extracts and infusions. 

 Bi. Cells, tissues or their derivatives used. 

 Teague and Deibert's Erythrocyte 



Agar 2142. 



Duval's Leprous Tissue Agar 2143 



Nastiukoff's Egg Yolk Agar (Recht- 



samer) 2144 



Steinschneider's Egg Yolk Agar 2145 



Brown's Albumen Agar (Giltner) . . . 2146 

 Scales' Ammonium Lactate Egg 



Agar 2147 



Scales' Egg Starch Agar 2148 



Despeignes' Egg Yok Milk Agar... 2149 



Noyes' Gelatin Agar 2150 



Standfuss and Kallert's Bone Jelly 



Agar 2151 



B2.* Body fluids used. 

 Ci. Blood employed. 



* See B3, page 691. 



