692 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



using sheep serum, or without the addi- 

 tion of serum. 



Variants: The authors added various 

 amounts of sheep or rabbit serum, heated 

 at 55°C. for 15 minutes to the medium as 

 prepared above. 



Reference: Teague and Deibert (1922 p. 

 70). 



2143. Duval's Leprous Tissue Agar 



Constituents: 



1. Agar solution. 



2. Tissue (Leprous). 

 Preparation : 



(1) Prepare an agar solution. Composi- 

 tion not given, but the author speci- 

 fied that it should contain no peptone. 



(2) Adjust to 1.5% alkaline. 



(3) Place small bits of leprous tissue upon 

 the surface of the sterilized and 

 solidified alkaline agar. 



(4) Moist the surface with a thin suspen- 

 sion of an organism capable of oxidiz- 

 ing nucleo-proteids such as B. typho- 

 sus, paraiyphosus, prodigiosus, py- 

 ocyaneus, dysenteriae, etc. 



(5) Incubate at 32°C. to 35°C. for two 

 weeks. 



Sterilization: Sterilization of agar not 



given. 

 Use: Indirect culture of B. leprae. 

 Reference: Duval (1911 p. 371). 



2144. Nastiukoflf's Egg Yolk Agar 

 (Rechtsamer) 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Egg yolk 100.0 cc. 



3. Agar 15.0 to 20.0 g. 



Preparation : 



(1) Separate the yolk of egg from the 

 white by Bunge's method (allow the 

 whites to roll from a blotting paper 

 containing the egg). 



(2) Add 0.5 cc. of a 10.0% NaOH solution 

 and 100.0 cc. of egg yolk to 1000.0 cc. 

 distilled water. 



(3) Add 15.0 to 20.0 g. of agar to (2) and 

 boil until there is complete solution 

 of the agar. 



(4) Allow to stand two hours in the 

 steamer. 



(5) Filter the thick liquid thru a hot 

 water funnel. 



(6) Distribute into sterile test tubes. 



Sterilization: Sterilize in the steamer. 

 Use: Cultivation of diphtheria, cholera, 



influenza and other pathogenic forms. 

 References: Nastiukoff (1893 ;^33 and 34), 



Rechtsamer (1895 p. 493). 



2145. Steinschneider's Egg Yolk Agar 

 Constituents: 



1. Distilled water 800.0 cc. 



2. Egg yolk 100.0 cc. 



3. Agar (2.0% soln.) 

 Preparation : 



(1) Mix two parts of sterile distilled water 

 with one part hen or lapwing's egg 

 yolk obtained under sterile con- 

 ditions. 



(2) Prepare a 2.0% agar solution. 



(3) Mix one part (1) with two parts (2). 



(4) Distribute into tubes. 



(5) Slant and solidify. 

 Sterilization: Final sterilization not given. 

 Use: Cultivation of gonococcus. The 



medium was not transparent. 

 Variants: The author prepared a similar 

 medium as follows: 



(1) Mix one part yolk of hen or lapwing's 

 egg with three parts sterile distilled 

 water. Shake thoroly. 



(2) To 20.0 g. of (1) add 10.0 cc. of a 20.0% 

 Na2HP04 and 3 times, hence 90.0 

 cc. of a 2.5 to 3.0% agar solution. 



(3) Pour into tubes and solidify (method 

 not given). 



(4) Final sterilization not specified. 

 The author reported that this medium 

 was not yellow and was nearly trans- 

 parent. Gonococcus growth was not 

 quite so luxuriant on this medium as on 

 serum agar. 



Reference: Steinschneider (1897 p. 380). 



2146. Brown's Albumin Agar (Giltner) 



Constituents : 



1. Distilled water 1000.0 cc. 



2. K2HPO4 0.5 g. 



3. MgS04 0.2 g. 



4. Egg albumin (powdered). . . 0.1 g. 



5. Glucose 10.0 g. 



6. Fe2(S04)3 trace 



7. Agar 15.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6, and 7 in 1. 



(2) Do not filter. 

 Sterilization: Not specified. 



