CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



693 



Use: To determine the bacterial count of 



soil. See medium 1439. 

 Reference: Giltner (1921 p. 373). 



2147. Scales' Ammonium Lactate Egg Agar 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Agar 15.0 g. 



3. Na2HP04 2.0 g. 



4. Ammonium lactate 10.0 cc. 



5. CaCOs 10.0 g. 



6. Egg 

 Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Titrate to +3. 



(3) Dissolve 4 and 5 in (2). 



(4) Add 2.0 cc. of whole egg to 7.0 cc. of 

 (3). 



(5) Tube. 



Sterilization: Sterilize at 12 pounds pres- 

 sure for 15 minutes. 



Use: To study variation in morphology of 

 B. coli. 



Reference: Scales (1921 p. 595). 



2148. Scales' Egg Starch Agar 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Starch (soluble 0.1%) 1.0 g. 



3. Agar (1.5%) 15.0 g. 



4. Eggs 

 Preparation : 



(1) Strain a fresh whole egg thru four 

 thicknesses of cheese cloth four times. 



(2) Prepare a soluble solution of agar 

 1.5%, and 0.1% soluble starch in 

 distilled water 



(3) Add 2.0 cc. of (1) to each 7.0 cc. of 

 (2). 



Sterilization: Sterilize at 12 pounds pres- 

 sure for 15 minutes. 



Use: To study variations in morphology 

 of B. coli. 



Reference: Scales (1921 p. 596). 



2149. Despeignes' Egg Yolk Milk Agar 



Constituents: 



1. Distilled water 



2. Milk 100.0 cc. 



3. Glycerol 15.0 g. 



4. Agar 9.0 g. 



5. Gentian violet 



6. Egg Yolk 



Preparation : 



(1) Add 100.0 cc. of boiled skim milk, 

 15.0 g. glycerol and 9.0 g. of agar to 

 200.0 cc. of Besredka's egg yolk 

 solution (see variant of medium 1244) 



(2) Heat in a salt water bath until the 

 agar is dissolved. 



(3) Sterilize in the autoclave at 105°C. 

 (Time not specified.) 



(4) Add 3.0 cc. of 1.0% gentian violet 

 solution when cool. 



(5) Distribute in sterile tubes under 

 aseptic conditions. 



(6) Slant. 



Sterilization: Heat on 3 successive days at 



55 to 57°C. for 30 minutes. 

 Use: Isolation tubercle bacilli. 

 Reference: Despeignes (1922 p. 120). 



2150. Noyes' Gelatin Agar 



Constituents: 



1. Water ^ 1000.0 cc. 



2. Agar (best) 15.0 g. 



3. Gelatin 7.5 g. 



Preparation : 



(1) Dissolve 2 and 3 in 1. 

 Sterilization: Not specified. 

 Use: Cultivation of soil bacteria. Weiss 



used a similar medium to determine the 



bacterial count of water. 

 Variants : 



(a) Noyes added 1.0 or 2.0 g. starch. 



(b) Weiss prepared a similar medium as 

 follows: 



(1) Dissolve 15.0% gelatin in 500.0 cc. 

 of water from the "Mlihlgraben der 

 Schwarzawa." 



(2) Dissolve 1.5% agar in 500.0 cc. 

 water. 



(3) Mix (1) and (2). 



References: Noyes (1916 p. 93), Weiss (1920 

 p. 25). 



2151. Standfuss and Kallert's Bone Jelly 

 Agar 



Solidify medium 1360 with agar. 



2152. Nicolle's Blood Agar 



Constituents : 



1. Water 900.0 cc. 



2. Agar 14.0 g. 



3. NaCl 6.0 g. 



4. Blood, rabbit 



