700 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



2165. Harvey's Nutrose Agar 

 Constituents : 



1. Water 1000.0 cc. 



2. Agar 15.0 g. 



3. Nutrose 15.0 g. 



Preparation : 



(1) Dissolve 15.0 g. of nutrose (or soma- 

 tose) and 15.0 g. agar in 1000.0 cc. of 

 water. 



Sterilization: Not specified. 



Use: Cultivation of amoebae. 



Reference: Harvey (1921-22 p. 97). 



2166. de Kruyff's Manure Agar 

 Constituents: 



1. Distilled water 1000.0 cc. 



2. Agar 20.0 g. 



3. K2HPO4 0.1 g 



4. NH4NO3 0.5 g. 



5. Stable manure 50.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. Details 

 of method of preparation not given). 

 Sterilization: Not specified. 

 Use: Cultivation of Myxococcus javan- 



ensis. 

 Reference: de Kruyff (1908 p. 385). 



2167. Henssen's Organ Infusion Agar 

 Constituents : 



1. Water 200.0 cc. 



2. Kidney 100.0 g. 



3. Agar 2.5 g. 



Preparation : 



(1) Remove the capsule from the kidney 

 (dog, calf and hog were used) and rub 

 to a pulp in a mortar with an equal 

 amount of water. 



(2) Allow to extract for 3 hours. 



(3) Press thru fine pored linen. 



(4) Prepare a 2^% water solution of agar. 



(5) Heat(4)to40°C. 



(6) Mix equal parts of sterile (5) and 

 sterile melted agar cooled to 40°C. 



(7) Incubate to test sterility. 

 Sterilization: Filter (3) thru a sterile clay 



filter using a water suction pump to 

 facilitate the filtering. Method of ster- 

 ilization of agar not given. 

 Use: To study the effect of kidney infusion 

 on the growth of some organisms. The 

 author reported that the medium checked 

 the growth of the organisms studied, i.e., 

 diphtheria bacilli, anthrax bacilli, B. coli, 



typhoid and cholera organisms. Livin- 

 good used a similar medium using B. coli, 

 B. typhosus, B. anthracis, B. diphtheriae, 

 B. pseudodiphtheria, and reported that 

 liver gave the best general results. 

 Variants: Livingood prepared a similar 

 medium as follows: 



(1) Pass 1 pound of organs (liver, beef 

 and hog, or spleen, sheep and hog, or 

 or adrenals, beef and sheep) thru a 

 meat chopper and macerate with 

 1000.0 cc. of tap water for 12 hours 

 on ice. 



(2) Press the juice thru a sterile towel. 



(3) Force the fluid thru a Chamberland 

 filter using carbonic acid pressure and 

 collect in a sterile flask. 



(4) Prepare a 2.0% agar solution (no 

 NaCl or peptone) sterilize. (Bouil- 

 lon agar, with NaCl and peptone, 

 maybe used.) 



(5) Mix equal parts of melted (4) and 

 (3). 



(6) Incubate for 24 to 48 hours to deter- 

 mine sterility. 



References: Henssen (1895 p. 403), Livin- 

 good (1898 p. 981). 



2168. Mayer's Salivary Gland Agar 



Constituents: 

 1- Water 500.O cc. 



2. Salivary glands 500.0 g. 



3. Agar (1.5%) 15.O g. 



Preparation : 



(1) Chop fresh salivary glands in a meat 

 chopping machine. 



(2) Mix with an equal weight of water. 



(3) Infuse on ice for 24 hours after strong 

 stirring. 



(4) Press the mass in a pressing machine. 



(5) Solidify sterile (6) by the addition of 

 1.5% agar (method not given). 



Sterilization: Sterilize (4) in streaming 

 steam for 30 minutes. Final sterilization 

 not specified. 



Use: General culture medium. Author re- 

 ported that meat extract media generally 

 gave better results. 



Reference: Mayer (1899 p. 747). 



2169. Graham-Smith's Heart Infusion Agar 



Solidify medium 1342 by the addition of 

 agar. 



