CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



701 



2170. Kutscher's Meat Infusion Agar 



Constituents : 



1. Meat water 1000.0 cc. 



2. Agar (1.5%) 15-0 g. 



Preparation : 



(1) Prepare meat water. 



(2) Dissolve 15.0 g. agar in sterile (1) by 

 boiling over a free flame. 



(3) Make slightly alkaline by the addition 

 of soda solution. 



(4) Filter. 



Sterilization: Method of sterilization of 

 meat water not given. Method of 

 sterilization of the final medium not 

 given. 



Use: Isolation and cultivation of Spirillum 

 iindula majris. Meier used a similar me- 

 dium to determine the numbers of bac- 

 teria in milk and milk products. 



Variants: Meier prepared a medium as 

 follows: 



(1) Boil 500.0 g. of fat and tendon free 

 beef in 1 liter water. 



(2) Filter. 



(3) Dissolve 15.0 g. agar and 5.0 g., 20.0 

 g. or 40.0 g. lactose or glucose in the 

 filtrate. 



(4) Neutralize by the addition of KOH. 

 ■ Add KOH until turmeric paper is 



turned quite weakly brownish red. 



(5) Sterilization not specified. 

 References: Kutscher (1895 p. 615), Meier 



(1918 p. 436). 



2171. Gassner's Basal Fuchsin Infusion 

 Agar 



Constituents : 



1. Water 750.0 cc. 



2. Meat infusion 250.0 cc. 



3. Agar 30.0 g. 



4. NaCl 5.0 g. 



5. Fuchsin (acid) (3.0% soln.).. 60.0 cc. 

 Preparation : 



(1) Prepare meat infusion (method not 

 given). 



(2) Mix 750.0 cc. of water with 250.0 cc. 

 of (1). 



(3) Dissolve 30.0 g. agar and 5.0 g. NaCl 

 in (2). 



(4) Adjust to a slight alkalmity usmg 

 litmus as an indicator. 



(5) Dissolve one of the added nutrients 

 in (4). 



(6) To each 80.0 cc. of (5) add 5.0 cc. of a 

 3.0% acid fuchsin solution and 0.0, 

 0.1, 0.2, 0.5, or 1.0% glucose. 



Sterilization: Not specified. 



Use: To study nitrogen source require- 

 ments for colon-typhoid and dysentery 

 group. The author reported that as- 

 paragin was utilized as a nitrogen source 

 by the typhoid and dysentery group as 

 well as by Bacterium coli. 



Added nutrients: The author added 10.0 g. 

 asparagin or 20.0 g. peptone. 



Variants: The author added 0.1, 0.2, 0.5, 

 or 1.0%, glucose. See step (6) above. 



Reference: Gassner (1917-18, p. 263). 



2172, Harde and Hauser's Fish Infusion 

 Agar 



Same as medium 1346, but solidified by 

 the addition of 15.0 g. agar. 



2173. Pergola's Mussel Infusion Agar 

 Solidify medium 1347 by the addition of 



2.0%, agar. 



2174. Harvey's Glucose Starch Infusion 

 Agar 



Constituents : 



1 Water 1500.0 cc. 



2. Beef 500.0 g. 



3. Agar 23.0 g. 



4. Starch 15-0 g. 



5. Glucose 15.0 g. 



6. Litmus solution 

 Preparation: 



(1) Macerate 500.0 g. finely minced beef 

 in 1500.0 cc. water in the ice chest 

 over night. 



(2) Filter thru doubled gauze. 



(3) Bring the filtrate to the boil and add 

 agar 1.5%. 



(4) Boil 25 minutes. 



(5) Estimate and adjust reaction to 0.5% 

 acid to phenolphthalein. 



(6) Clarify and filter. 



(7) Add 1.0%, starch, 1.0%o glucose and 

 color the medium with litmus solu- 

 tion. 



(8) Steam 45 minutes shaking 3 times 

 during this period to distribute the 

 starch. 



(9) Distribute into test tubes. 

 Sterilization: Sterilize 15 minutes at 10 



pounds. 



