702 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Use: Differentiation of meningococci and 

 M. catarr halts. Author reported that 

 meningococci acidified with glucose, M. 

 catarrhalis did not. 



Reference: Harvey (1921-22 p. 111). 



2175. Vedder's Starch Infusion Agar 

 Constituents: 



1. Beef infusion (not 

 containing peptone 



or salt) 1000.0 cc. 



2. Starch, corn 10.0 g. 



3. Agar 15.0 to 17.5 g. 



Preparation ; 



(1) Exact composition of beef infusion 

 not given except that it should con- 

 tain no peptone or salt. 



(2) Dissolve agar in beef infusion, cook, 

 clarify and filter according to methods 

 of preparing ordinary agar. 



(3) Neutralize so that final reaction will 

 be from 0.2 to 0.5% acid to phenol- 

 phthalein. 



(4) Grind corn starch in mortar with a 

 little of (3) to avoid lumps. 



(5) Boil (4) for a few minutes. 



(6) Tube. 



Sterilization: Sterilize at not more than 15 

 pounds pressure. 



Use: Special culture medium. Author re- 

 ported that medium will keep gonococci 

 alive for long period of time. May be 

 used to isolate gonococci and for routine 

 purposes. Park, Williams and Krum- 

 wiede recommended a similar medium 

 for the cultivation of meningococci. 



Variants : 



(a) Harvey prepared the medium as 

 follows: 



(1) Macerate 500.0 g. finely minced 

 beef in 1500.0 cc. water in the ice 

 chest over night. 



(2) Filter thru doubled gauze. 



(3) Bring the filtrate to the boiling 

 point and add 1.5% agar. 



(4) Boil 25 minutes. 



(5) Estimate and adjust reaction to 

 0.5% acid to phenolphthalein. 



(6) Clarify and filter. 



(7) Add 1.0% starch. 



(8) Steam 45 minutes shaking 3 times 

 during this period to distribute the 

 starch. 



(9) Distribute into test tubes. 

 (10) Sterilize 15 minutes at 10 pounds 

 pressure. 

 He reported that there should be an 

 abundance of water of condensation 

 developed, and the agar must not 

 exceed 1.5%. 

 (b) Park, Williams and Krumwiede pre- 

 pared a medium as follows: 



(1) Extract 500.0 g. beef in 1000.0 cc. 

 of water in an ice box over night. 



(2) Boil and strain. 



(3) Dissolve 1.5% agar in (2). 



(4) Adjust the reaction to -1-0.2 to 

 -hO.7 to phenolphthalein (pH be- 

 tween 7.0 and 7.6). 



(5) Cool and clarify with eggs. 



(6) Filter. 



(7) Add 1.0% starch and heat in the 

 Arnold for 45 minutes shaking the 

 mediimi several times to distribute 

 the starch. 



(8) Tube. 



(9) Autoclave 15 minutes at 10 pounds 

 pressure. 



References: Vedder (1914 p. 385), Tanner 

 (1919 p. 60), Giltner (1921 p. 371), Harvey 

 (1921-22 p. HI), Park, Williams and 

 Krumwiede (1924 p. 131). 



2176. Kopp's Thyroid Agar 



Constituents : 



1. Water 1000.0 cc. 



2. Thyroid gland, sheep 500.0 g. 



3. Agar lO.O g. 



4. Glycerol 30.0 cc. 



5. NaCl 5.0 g. 



Preparation : 



(1) Free sheep thyroid glands from fat, 

 and chop the glands into small pieces. 



(2) Extract the glands for 3 hours with an 

 equal weight of sterile water. 



(3) Add (2) to moistened linen and press 

 the fluid thru. 



(4) Dissolve 3, 4 and 5 in 500.0 cc. water. 



(5) Mix equal parts of sterile (4) and 

 sterile (3) melted and cooled to 40°C. 



Sterilization: Filter (3) thru a clay filter to 

 sterilize. Method of sterilization of 

 agar not given. 



Use: Cultivation of colon-typhoid group. 



Reference: Kopp (1895 p. 81). 



