708 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



(1901 p. 728), (1902 pp. 151, 152, 156), 

 Frost (1903 p. 340), Ball (1919 p. 80), 

 Tanner (1919 pp. 51, 56) Heinemann 

 (1922 p. 34). 



2192. Stapp's Egg White Extract Agar 



Constituents: 



1. Water 500.0 cc. 



2. Egg white 



3. Meat extract 1.33 g. 



4. NaCl 0.33 g. 



5. Glucose 1.66 g. 



6. Agar 10.0 g. 



Preparation : 



(1) Beat fresh egg white until it is a 

 completely uniform liquid. 



(2) Mix with water in the ratio of 1:100 

 by thoro shaking. 



(3) Close the flask and heat in the 

 steamer for 20 minutes. 



(4) When all the air is removed from the 

 flask place the flask in the autoclave 

 and heat at 138° for 20 minutes. 



(5) Remove after cooling and filter. 



(6) Mix 200.0 g. of the filtrate, 0.33 g. 

 NaCl, 1.66 glucose, 10.0 g. agar, 1.33 

 g. meat extract and 300.0 cc. water. 

 (The meat extract may be omitted). 



(7) Dissolve by heating in the steamer. 



(8) Filter while hot. 

 Sterilization: Not specified. 



Use: Cultivation of Bac. cobayae, Bac. 



capri, Bac. guano, Bac. musculi, Bac. 



hollandicus. 

 Reference: Stapp (1920 p. 5). 



SUBGROUP II-C. SECTION 10 



Basal or complete media containing agar 

 with derivatives of soil, ashes, etc., but not 

 containing digests. 

 Ai. Ashes or derivatives employed. 

 Harrison and Barlow's Wood Ash 



Agar 2193 



A2. Soil infusions or extracts employed. 



Fremlin's Soil Infusion Agar 2194 



Gowda's Soil Infusion Agar 2195 



Conn's Soil Infusion Agar 2196 



Lohnis' Mannitol Soil Infusion 



Agar 2197 



Perotti's Dicyandiamide Soil Infu- 

 sion Agar 2198 



2193. Harrison and Barlow's Wood Ash Agar 



Constituents: 



1. Water 200.0 cc. 



2. Ashes, wood 5.0 g. 



3. Agar 2.0 g. 



4. Maltose 2.0 g. 



Preparation : 



(1) Dissolve 2.0 g. agar and 2.0 g. maltose 

 in 100.0 cc. water by heat. 



(2) Add 5.0 g. wood ashes to 100.0 cc. 

 water. 



(3) Boil and filter. 



(4) Mix 100.0 cc. of (1), 40.0 cc. of (2), 

 and 60.0 cc. of water or mix 100.0 

 cc. (1) with 60.0 cc. (2) and add 40.0 

 cc. of water or mix 100.0 cc. (1) with 

 100.0 cc. of (2) and add no water. 



Sterilization: Sterilize in streaming steam 



or in the autoclave at 10 pounds for 10 to 



20 minutes. 

 Use: Cultivation of Pseudornonas radici- 



cola and other bacteria from the nodules 



of leguminous plants. 

 Variants : 



(a) Harrison and Barlow gave a number 

 of different media, using varying 

 amounts of constituents, as in 

 medium 1377, and then adding 1.2, 

 1.5 or 2.0% agar to solidify. 



(b) Lohnis prepared the medium as 

 follows: 



(1) Infuse 4.0 g. of wood ashes with 

 1000.0 cc. of water. 



(2) Add 4.0% maltose and 2.0% 

 K2HPO4 to (1). 



(3) Solidify with agar. 



(4) Sterilization not specified. 



(c) Harvey dissolved 4.0 g. maltose, 5.0 

 g. wood ashes and 10.0 g. agar in 

 1000.0 cc. distilled water. 



(d) Percival prepared the medium as 

 follows: 



(1) Add 8.0 g. of well burnt wood ashes 

 to 500.0 cc. distilled water and boil 

 for one minute. 



(2) Filter thru two sheets of paper. 



(3) Add 4.0 g. maltose and 4.0 g. of 

 agar to (2). 



(4) Heat until dissolved. 



(5) Filter. 



(6) Tube. 



(7) Sterilize in the usual way, method 

 not given. 



(8) Slant. 



(e) Giltner gave the following method of 

 preparation: 



(1) Stir 5.0 g. of wood ashes (elm, 

 beech, maple) into 1000.0 cc. of 



