710 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Variants: Harvey prepared a similar me- 

 dium as follows: 



(1) Dissolve 15.0 g. agar in 900.0 cc. of 

 water. 



(2) Boil 2000.0 cc. water and 1000.0 g. 

 garden soil for 2 hours. 



(3) Pour off the turbid fluid. 



(4) Mix talc with (3). 



(5) Filter until clear thru thick filter 

 paper. 



(6) Mix 100.0 cc. of (5) and 900.0 cc. of 



(1). 



(7) The reaction should be 0.5% acid to 

 phenolphthalein. 



(8) Add 1.0 g. glucose to (7). 



(9) Tube. 



(10) Sterilization not specified. 

 References: Tanner (1919 p. 49), Harvey 

 (1921-22 p. 105). 



2197. Lohnis' Mannitol Soil Infusion Agar 



Constituents : 



1. Water 1000.0 cc. 



2. Soil 1000.0 g. 



3. Mannitol (1.0%) 10.0 g. 



4. K2HPO4 (0.05%) 0.4 g. 



5. Agar (1.5%) 12.0 g. 



Preparation : 



(1) Heat 1000.0 g. of good rich garden 

 soil with a liter of tap water for 30 

 minutes in the autoclave under pres- 

 sure of 1 atmosphere or boil with 2 

 liters of water over a free flame. 



(2) Pour off the turbid liquid. 



(3) Mix talc with the liquid. 



(4) Filter thru a double filter paper. 



(5) Make up the volume to 800.0 cc. if 

 necessary. 



(6) Dissolve 3, 4 and 5 in (5). 



(7) Tube. 



Sterilization: Sterilize in the autoclave or 



stQamer. 

 Use: Isolation of nodule bacteria. 

 Reference: Lohnis (1913 p. 112). 



2198. Perotti's Dicyandiamide Soil Infusion 

 Agar 



Same as medium 1392 but solidified by the 

 addition of 1.5% agar. ^ 



