GROUP III. MEDIA SOLIDIFIED BY ADDITION OF GELATIN 



SUBGROUPS OF LIQUEFIABLE sporogenes, Bacillus hotulinus. Other 



GELATIN MEDIA investigators used similar media for a 



-, , , • • • . • 1 iu variety of purposes. 

 Ai. Xot containme organic materials other 



, , . ^ <= Variants: 



than gelatin Kufferath used a 10.0, 20.0, 30.0, 



Subgroup III-A (Med. 2199-2204) .n ^ -rr, r> i ^- ^ I- 



, r^ , . ■ ■ X ■ 1 • jj- 40.0 or 70.0 gelatin solution. 



As. Containing organic material m addi- /, s ,, j mnrv i x- i x- 



. ^ , ^ . (b) Meyer used a 10.0% gelatin solution, 



tion to gelatin j- x j i. n ^or / xx xx ix- 



° , TTT Ti /AT J none ooTiN adjustcd to 0.4% to attcmpt to culti" 



Subgroup III-B (Med. 2205-2371) [ . '." ^ ' 



vate the causative agent oi symptom- 



*^ STTRPROUP TTT-A atica anthra.x, or blackleg in swine. 



(c) Conn (1916) cultivated spore forming 



Gelatin Media, with Additional bacteria on a 12.0% gelatin in tap 



Constituents Inorganic water. The medium was clarified 



with white of egg. 



SUBGROUP III-A (d) Conn (1917) cultivated soil micro- 



r^ , , , J. . . • 1 organisms in a medium prepared by 



Basal or complete media containing gela- ,• , • or>r> n r V. u t u i 



.j,.^. , ,., , ., ° ° . dissolving 200.0 g. of Gold Label 



tin. Additional constituents, if any, in- , ^. . ,„^„ „ ^ ^ ,• x 



gelatin in 1000.0 cc. tap water, adjust- 



.„\.. ,,. , , , ing to 0.5% normal acid to phenol- 



Ai. Containing gelatin and water only. ° i • /or> n x on n e i 



' . u-x . /-I 1 x- oir»n phthalein (20.0 to 30.0 cc. of normal 



Natsuschita s Gelatin 2199 t'^ ^„, Jv x ixu xionn c 



_, , , o ix T-i r^ 1 x- ooriA NaOH). He reported that 120.0 g. of 



Taylor's Salt Free Gelatin 2200 ^ ^ ^ tt -x j ox x r^^ r^ 



--•' , -x . Ti 1 r-i 1 X- oor»i Bacto or United States Glue Co. 



Matzuschita s Basal Gelatin 2201 . . .,^, ,. ^ irr^u 



„ , . . ,, , ,, gelatin might be used instead or Gold 



A2. Containing added salts. ^ , , ^"i ,. _ . , 



„^ ^ J XT xi u> Tiu u X Label. Only 10.0 cc. of normal 



Stutzer and Hartleb's Phosphate .^ ___ -^ • , x • xu j 



„ , ^. r,r,nr> NaOH Were required to give the de- 



(jrelatin zzuz • i x- tx 



„, , , , ,,T^ ,, ,, nnrvo Sired Teaction. it was necessary to 



Stoklasa s "Kollagen' 2203 , .. ^, ,. , ■^ ., 



„.., , -- . „, , , clarify the medium, prepared with 



Sohngen's Magnesium Phosphate .... . ^, ^^ , „, ' J^ , x- u 



r^ ? J.- /Tr- 1- N oonA United States Glue Co. gelatin, by 



Gelatin (Vierling) 2204 ^, ,,.. . , -^ . , \ 



the addition oi white ot an egg, but 



2199. Matzuschita's Gelatin clarification was not necessary when 



Constituents: using Bacto gelatin. 



1. Water 1000.0 cc. (^) Committee S. A. B. gave the following 



2 Gelatin ' . . ' 100.0 g. method of preparation : 



Preparation: (1) Dissolve 120.0 g. Gold Label, 



(1) Thoroly shake 100.0 g. gelatin in one 100-0 §• B^^to, or 100.0 g. U. S. Glue 

 liter of water. ^^- gelatin in 1000.0 cc. distilled 



(2) Heat until the gelatin is melted. water. 



(3) Neutralize (indicator not specified). (2) Clarify with white of egg. 



(4) Boil in the steamer. (3) Adjust the pH between 6.6 and 7.4. 



(5) Filter. (4) Sterilize. 



Sterilization: Sterilize in the steamer on (0 Waksman studied the metabolism of 



from 2 to 5 successive days for 15 to actinomycetes in a 15.0% gelatin 



30 minutes. Incubate for 2 days at 37°C. solution, 



to test sterility. (g) Weiss dissolved 15.0% gelatin in 



Use: Used by Matzuschita for the cultiva- water and used the medium to de- 



tion of spore forming bacilli, Clostridium termine the bacterial count of water. 



hutyricum, Bacillus oedematis maligni, (h) Harvey cultivated bacteria found in 



Bacillus anthracis symptomatici, Bacillus soil on a medium prepared from one 



711 



