712 



CULTURE MEDIA FOR CULTIVATIOX OF MICRO ORGANISMS 



part gelatin to five parts of tap water. 



The reaction was adjusted to 0.5% 



acid to phenolphthalein. 

 References: Matzuschita (1902 p. 287), 

 Kufferath (1914 p. 559), Meyer (1915 

 p. 467), Conn (1916 p. 188), (1917 p. 42), 

 Committee S. A. B. (1918 p. 116), Waks- 

 man (1920 p. 22), Weiss (1920 p. 25), 

 Harvey (1921-22 p. 105), Committee 

 S. A. B. (1923 p. 10). 



2200. Taylor's Salt Free Gelatin 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Gelatin 100.0 g. 



Preparation : 



(1) Soak gelatin in weak alkali and after 

 it has become swollen, wash re- 

 peatedly by decantation. 



(2) Dissolve gelatin in water by heat. 



(3) Precipitate by the least effective 

 quantity of alcohol. 



(4) Wash with alcohol and ether and dry. 



(5) Dissolve 100.0 g. of (4) in 1000.0 cc. 

 of twice distilled water (conductivity 

 atl6°C. tobel.l X lO-^). 



(6) Submit the solution to electrolysis for 

 five days, the conductivity reaching a 

 constant (8 X 10-«). During electro- 

 lysis, dialysis is taking place at all 

 times by means of a special apparatus. 

 A 110 volt circuit was used. 



Sterilization: Not specified. 



Use: Salt free medium. The author re- 

 ported that the medium did not support 

 the growth of ordinary saprophytic or 

 pathogenic bacteria. 



Reference: Taylor (1905 p. 114). 



2201. Matzuschita's Basal Gelatin 



Constituents : 



1. Gelatin 10.0% 



Preparation: (1) Solidify one of the added 



nutrients by the addition of 10.0% 



gelatin. 

 Sterilization : Method not specified. 

 Use: Cultivation of intestinal bacteria. 

 Added nutrients: The author used one of 



the following: 



Beer wort 



Urine 



Straw Infusion 

 Reference: Matzuschita (1901-02 p. 214). 



2202. Stutzer and Hartleb's Phosphate 

 Gelatin 

 Constituents: 



1- Water looo.O cc. 



2. Gelatin lOO.O g. 



3. Potassium phosphate 1.0 g. 



Preparation : 



(1) Dissolve 100.0 g. of gelatin and 1.0 g. 

 of potassium phosphate in water. 



(2) Make up the volume to 1 liter. 



(3) Add soda to obtain a neutral reaction. 

 Sterilization: Method not specified. 



Use: Cultivation of Zoogloea ramigera, a 



nitrate fungus. 

 Reference: Stutzer and Hartleb (1897 



p. 236). 



2203. Stoklasa's "Kollagen" 

 Constituents : 



1- Water 1000.0 cc. 



2. "Kollagen" (c.p. with 

 17.02% N "Ossein") 



3. K.SO4 1.0 g. 



4. MgCl2 0.5 g. 



5. Iron sulphate 0.1 g. 



6. CaHP04 0.0 or 1.0 g. 



Preparation: (1) Dissolve 2, 3, 4, 5 and 6 



in 1. 

 Sterilization: Sterilize in the autoclave. 

 Use: To study XH3 formation by Bacillus 



mycoides, Bacillus suhtilis, Bacillus 



mesentericus vulgatus. 

 Reference: Stoklasa (1911 p. 476). 



2204. Sohngen's Magnesium Phosphate 

 Gelatin (Vierling) 



Constituents : 



1. Water 1000.0 cc. 



2. Gelatin lOO.O g. 



3. MgSO^. 1.0 g. 



4. K2HPO4 1.0 g. 



5. CaCU trace 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Pour sterile (1) into plates. 

 Sterilization: Not specified. 



Use: Cultivation of Mycobacteria. The 

 author reported that the plates were 

 slightly turbid due to a precipitate of 

 Mg3(P04)2. After 8 days mycobacteria 

 caused a clearing of the medium around 

 the colony. 



Reference: Vierling (1920 p. 202). 



