CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



13 



SUBGROUP III-B 



Gelatin Media, with Additional 

 Constituents Organic 



Key to the Sections of Subgroup III-B 



Ai. Chemical composition of all constit- 

 uents known . Section 1 (Med. 2205-2234) 

 A2. Chemical composition of one or more 



constituents not definitely known. 

 Bi. Containing digests 



Section 2 (Med. 2235-2323) 

 B2. Not containing digests 



Section 3 (Med. 2324-2371) 



SUBGROUP III-B. SECTION 1 



Basal or complete media containing 

 gelatin with additional organic materials 

 of known chemical composition. 

 Ai. No additional organic nitrogen used. 

 Bi. Inorganic nitrogen salts not added. 

 Ci. Containing carbohydrates. 



Beijerinck's Starch Gelatin 2205 



Waksman's Starch Gelatin 2206 



Dombrowski's Glucose Gelatin 2207 



Fraenkel's Glucose Glycerol Gelatin. 2208 

 Beijerinck's Acid Starch Gelatin.. 2209 

 BobiliofT-Preisser's Basal Glucose 



Gelatin 2210 



C2. Not containing carbohydrates; other 

 organic carbon used. 



Sohngen's Petroleum Gelatin 2211 



Weisser's Glycerol Gelatin 2212 



Proskauer and Beck's Glycerol Gela- 

 tin (Klimmer) 2213 



Fermi's Phenol Gelatin 2214 



B2. Inorganic nitrogen salts added. 

 Sohngen's Ammonium Chloride 



Malate Gelatin 2215 



Woltje's Basal Sucrose Gelatin 



(Zikes) 2216 



Mortensen's Sucrose Ammonium 



Sulphate Gelatin 2217 



Higgins' Lactose Ammonium Suc- 

 cinate Gelatin 2218 



V. Tubeuf's Sucrose Ammonium Ci- 

 trate Gelatin (Malenkovic) 2219 



Cohn's Ammonium Tartrate Gelatin 



(Klimmer) 2220 



Kossowicy's Ammonium Phosphate 



Sucrose Gelatin (Will) 2221 



V. Tubeuf's Citric Acid Ammonium 

 Nitrate Gelatin 2222 



Munter's Basal Glucose Salt Gela- 

 tin 2223 



Mortensen's Basal Nitrate Gelatin . . 2224 

 A2. Additional organic nitrogen employed. 

 B:. Containing carbohydrates. 

 Ci. Carbohydrates, only, added. 

 Beijerinck's Starch Asparagin Gela- 

 tin 2225 



C2. Carbohydrates and other organic car- 

 bon added. 



Grimbert's Starch Asparagin Gela- 

 tin (Charrin and Dissard) 2226 



Kappen's Cyanamide Asparagin 



Gelatin 2227 



Maassen's Glucose Asparagin Gela- 

 tin (Klimmer) 2228 



B2. Not containing carbohydrates. 

 Ci. Containing organic acids. 



van Delden's Lactate Asparagin 



Gelatin 2229 



Frankel's Lactate Asparagin Gela- 

 tin (Klimmer) 2231 



Uschinsky's Glycerol Asparaginate 



Gelatin (Klimmer) 2232 



C2. Not containing organic acids. 



Goslings' Asparagin Gelatin 2233 



Sullivan's Nitrate Asparagin Gela- 

 tin 2234 



2205. Beijerinck's Starch Gelatin 



Constituents : 



1. Water 1000.0 cc. 



2. Gelatin 100.0 g. 



3. KH2PO4 0.5 g. 



4. Starch (soluble) 1-0 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Pour into plates. 

 Sterilization: Not specified. 



Use: Detection of quinone production. 

 The author seeded the medium by pouring 

 a culture of S. chromogena in medium 250 

 over the plate. After a few days the 

 gelatin was melted and the quinone was 

 extracted with benzol. 



Reference: Beijerinck (1900 p. 10). 



2206. Waksman's Starch Gelatin 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Gelatin 150.0 g. 



3. Starch 10.0 g. 



