714 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Tube. 



Sterilization: Method not given. 



Use: To study metabolism of actinomy- 



cetes. 

 Reference: Waksman (1920 p. 22). 



2207. Dombrowski's Glucose Gelatin 

 Constituents : 



1- Water 1000.0 cc. 



2. MgSO, (0.1%) 1.0 g. 



3. K2HPO4 (0.2%) 2.0 g. 



4. Glucose (5.0%) 50.0 g. 



5. Gelatin (10.0%) 100.0 g. 



Preparation: (1) Dissolve 2, 3, 4 and 5 in 1. 

 Sterilization: Not specified. 



Use: Cultivation of milk yeasts. 

 Reference: Dombrowski (1910 p. 380). 



2208. Fraenkel's Glucose Glycerol Gelatin 

 Constituents : 



1. Water 1000.0 cc. 



2. Gelatin 150.0 g. 



3. Glycerol 2.5 g. 



4. Dextrose 2.5 g. 



Preparation: (1) Dissolve 2, 3 and 4 in 1. 

 Sterilization: Sterilize in the usual manner 



(method not specified). 

 Use: Cultivation of nitrate fungi. 

 Reference: Fraenkel (1898 p. 10). 



2209. Beijerinck's Starch Gelatin 

 Constituents : 

 1- Water 1000.0 cc. 



2. Gelatin 100.0 g. 



3. Lactic acid 



4. Starch 

 Preparation : 



(1) Dissolve 10.0% commercial gelatin 

 in water. 



(2) Acidify slightly with lactic acid (a few 

 drops). 



(3) Add a little starch (amount not 

 given). 



(4) Four into Petri dishes. 

 Sterilization: Not specified. 



Use: To test for quinone production by 

 Streptothrix chromogena. The author 

 reported that the gelatin turned brown 

 due to the oxidation of the formed 

 quinone. The acid hindered this oxida- 

 tion and stored the quinone. When KI 



dissolved in dilute HCl, was added the 

 gelatin was colored highly blue as far 

 as the quinone had diffused from the 

 colony. 

 Reference: Beijerinck (1900 p. 9). 



2210. Bobilioflf-Preisser's Basal Glucose 

 Gelatin 

 Constituents : 



1. Distilled water 1000.0 cc. 



2. Hayduck's Mineral Solution 20.0 cc. 



3. Glucose 50.0 g. 



4. Gelatin (10.0%) IQO.O g. 



Preparation : 



(1) Composition of Hayduck's Mineral 

 Solution not given. 



(2) Add 20.0 cc. of (1) to 1000.0 cc. of 

 distilled water. 



(3) Dissolve 3, 4 and one of the added 

 nutrients in (2). 



(4) Distribute in 10.0 cc. lots in small 

 Erlenmeyer flasks. 



Sterilization: Sterilize in the steamer. 



Use: Cultivation of fungi imperfecti, 

 oospora. The author reported that the 

 growth was inhibited using inorganic 

 nitrogen source. The gelatin was lique- 

 fied. 



Added nutrients : The author added one of 

 the following materials: 



Asparagin 2.5 g. 



Peptone 21g 



(NH4)2S04 2.5 g.' 



Reference: Bobilioff-Preisser (1916 p. 395). 



2211. Sohngen's Petroleum Gelatin 

 Same as medium 1460 but solidified by 

 the addition of 10.0% gelatin instead of 

 2.0% agar. 



2212. Weisser's Glycerol Gelatin 

 Constituents : 



1. Water 1000.0 cc. 



2. Gelatin (10.0%) 100.0 g. 



3. Glycerol (2.0%') 20.0 g. 



Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Adjust the reaction to a slight alka- 

 linity. 



Sterilization: Not specified. 

 Use: Cultivation of Neapler cholera bac- 

 teria (Emmerich). 

 Reference: Weisser (1886 p. 326). 



