CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



715 



2213. Proskauer and Beck's Glycerol 

 Gelatin (Klimmer) 



Constituents: 



1. Water 1000.0 cc. 



2. Glycerol 15.0 g. 



3. (NH4)2C03 3.5 g. 



4. MgS04 (Crystalline) 2.5 g. 



5. KH2PO4 1-3 g. 



6. Gelatin 100.0 to 150.0 g. 



Preparation: (1) Dissolve 2, 3, 4, 5 and 6 



in 1. 

 Sterilization: Not specified. 

 Use: Cultivation of tubercle bacilli. 

 Reference: Klimmer (1923 p. 172). 



2214. Fermi's Phenol Gelatin 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Gelatin (10.0%) 100.0 g. 



3. Phenol (0.5%) 5.0 g. 



4. Na.COa (1.0 or 2.0%) 

 Preparation : 



(1) Dissolve the gelatin in 1 at 80 to 90°C. 



(2) Add 3 and 4 to (1). 



(3) Adjust the reaction as desired, by the 

 addition of soda, mineral or organic 

 acids. 



(4) Distribute in tubes. 

 Sterilization: Not specified. 



Use: Prove proteolytic enzyme. The 

 author reported that if a proteolytic en- 

 zyme was present the gelatin was 

 liquefied. 



Variants: The author used 1.0, 2.0 or 5.0% 

 gelatin instead of 10.0%. 



Reference: Fermi (1906 p. 177). 



2215. Sohngen's Ammonium Chloride 

 Malate Gelatin 



Constituents : 



1. Water 1000.0 cc. 



2. K.,HP04 (0.05%) 0.5 g. 



3. NH4CI (0.05%) 0.5 g. 



4. Calcium malate (0.5%) 5.0 g. 



5. Gelatin (10.0%) 100.0 g. 



Preparation: (1) Dissolve 2, 3, 4 and 5 in 1. 

 Sterilization: Not specified. 



Use: Cultivation of urea splitting 



organisms. 

 Reference: Sohngen (1909 p. 94). 



2216. Woltje's Basal Sucrose Gelatin 



(Zikes) 



Constituents : 



1. Water 1000.0 cc. 



2. K2HPO4 5.0 g. 



3. MgS04 2.5 g. 



4. Sucrose 75.0 g. 



5. Gelatin 140.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Dissolve one of the added nutrients 

 in (1). 



Sterilization: Not specified. 



Use: To study perithezien formation by 



Aspergillus oryzae. 

 Added nutrients: The author added 10.0 g. 



of peptone, 10.0 g. asparagin, 10.0 g. 



(NH 4)2804 to the basal medium. 

 Variants : Zikes used 5.0 g. K2SO4 instead of 



K2HPO4, used 140.0 g. gelatin and used 



10.0 g. asparagin as an added nutrient. 

 References: Zikes (1922 p. 342). 



2217. Mortensen's Sucrose Ammonium 



Sulphate Gelatin 

 Same as medium 207 but solidified by the 

 addition of gelatin. 



2218. Higgins' Lactose Ammonium 

 Succinate Gelatin 



Constituents : 



1. Water 



2. Glucose 



3. Lactose 



4. Ammonium succinate. 



5. (NH4)2S04 



6. KCl 



7. KNO3 



8. Ammonium phosphate 



1000.0 cc. 

 2.5 g. 

 2.5 g. 

 2.5 g. 

 1.0 g. 

 1.5 g. 

 2.5 g. 

 2.5 g. 



9. Gelatin (7.0%) 70.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6, 7, 8 and 9 in 1. 



(2) Neutralize to phenolphthalein. 

 Sterilization: Method not given. 



Use: Cultivation of organisms causing 

 cholera, Pictoy cattle disease and others. 

 Reference: Higgins (1898 p. 668). 



2219. v. Tubeuf's Sucrose Ammonium 

 Citrate Gelatin (Malenkovic) 



Constituents: 



1. Water 1000.0 cc. 



2. K2HPO4 5.0 g. 



