720 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



12. Body fluids added. 



Pergola's Alkaline Blood Gelatin. . . 2319 



Muller's Blood Gelatin 2320 



Mailer's Serum Gelatin 2321 



13. Other animal derivatives added. 



A2. No commercial digest employed. Non- 

 commercial digest used. 

 Beijerinck's Trypsmized Gelatin... 2322 

 Jensen's Pepsinized Milk Gelatin. . . 2323 



2235. Will's Basal Salt Peptone Gelatin 



Same as medium 583, but solidified by the 

 addition of 10.0% gelatin. The author also 

 solidified the basal solution, see medium 

 555, by the addition of 10.0% gelatin. 



2236. Matzuschita's Basal Peptone 

 Gelatin 



Same as variant (b) medium 568, but 

 solidified by the addition of 10.0% gelatin. 

 References: Kita (1913 p. 446), Mat- 



zuschita (1902 p. 286, 288). 



2237. Banning's Basal Peptone Gelatin 



Same as medium 1535, but solidified by 

 the addition of 70.0 g. gelatin instead of 

 10.0 g. agar. 



2238. Harvey's Basal Neutral Red Peptone 



Gelatin 

 Constituents : 



1- Water 1000.0 cc. 



2. Peptone 20 g 



3. KOH(5.0%) 10.0 cc. 



4. Gelatin 75.O g. 



5. Neutral red (1.0%) (0.5%) . . 5.0 cc. 

 Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Steam 45 minutes. 



(3) Filter. 



(4) Add 5.0% of a 20.0% solution of one 

 of the added nutrients, and 0.5% of a 

 1.0% neutral red solution. 



Sterilization: Sterilize for 10 minutes on 

 each of 3 successive days at 100°C. 



Use: Cultivation and differentiation of 

 colon group. 



Added nutrients: The author added 5.0% 

 of a 20.0% solution of any desired sugar. 



Reference: Harvey (1921-22 p. 109). 



2239. Weiss' Nahrstoff Heyden Gelatin 

 Constituents : 

 1- Water 1000.0 cc. 



2. Gelatin (15.0%) 150.0 g. 



3. Nahrstoff Heyden (0.8%) ... 8.0 g. 

 Preparation: (1) Dissolve 2 and 3 in 1. 

 Sterilization: Not specified. 



Use: Bacterial count of water. The author 

 reported that generally an alkaline me- 

 dium gave a slightly higher bacterial 

 count. 



Reference: Weiss (1920 p. 25). 



2240. Jacobi's Peptone Gelatin 



Constituents: 



1- Water 1500.O cc. 



2. Meat peptone (Kem- 

 merich's) 22.5 g. 



3. Peptone (siccum) 45.0 g. 



4. Gelatin 225.0 g. 



Preparation : 



(1) Add 2 and 3 in 1. 



(2) Boil over a free flame for a minute. 



(3) Cool to about 50 to 60°C. 



(4) Dissolve the gelatin in (3) without 

 further heating. 



(5) Correct the acid reaction by the 

 addition of concentrated Na2C03. 



(6) Transfer to a large flask. 



(7) Add the white of an egg, mi.x well 

 and steam for | hour. 



(8) Filter, using compressed air to 

 hasten filtration. 



(9) Distribute in 50 to 100.0 cc. lots in 

 flasks. 



Sterilization: Place in flowing steam for 

 10 minutes on 3 successive days. 



Use: General culture medium. Other in- 

 vestigators used similar media for spe- 

 cific purposes as indicated below. 



Variants : 



(a) Jacobi stated that the medium might 

 be diluted with distilled water when 

 ready for use. 



(b) Matzuschita dissolved 10.0 g. of 

 Koch's peptone in 1000.0 cc. water 

 and solidified with gelatin. He culti- 

 vated spore forming bacilli on this 

 medium. 



(c) Lieske isolated iron bacteria, Lepto- 

 thrix ochracea on a medium made 

 slightly alkaline by the addition of 

 KOH containing 100.0 g. of gelatin 

 and 0.25 g. manganese peptone per 

 1000.0 cc. of peat water. 



(d) Weiss used 1.0% peptone instead of 



